沙土鼠及大鼠局部脑缺血时血小板活化因子的变化

Platelet-activating Factors in Local Cerebral Ischemia of Gerbils and Rats

为了探讨脑缺血后血小板活化因子（ｐｌａｔｅｌｅｔ－ａｃｔｉｖａｔｉｎｇｆａｃｔｏｒ，ＰＡＦ）含量的变化，采用沙土鼠单侧脑缺血和大鼠局部脑缺血模型，用薄层层析（ＴＬＣ）结合生物法测定了缺血后脑组织ＰＡＦ的含量结果：（１）沙土鼠右侧颈总动脉结扎３ｈ再灌２ｈ，右侧大脑组织ＰＡＦ含量较左侧明显升高，分别为（４４０±０９０）和（１３０±０４２）ｐｇ／ｇ（Ｐ＜００５）；（２）大鼠左侧大脑中动脉（ＭＣＡ）凝闭后４８ｈ，左侧大脑组织ＰＡＦ含量右侧亦明显升高。

To explore the release of platelet-activating factors (PAF), an ischemia model was established with the unilateral stroke in gerbils and with the regional cerebral ischemia in rats. Thin layer chromatography (TLC) was combined with biological methods for the determination of PAF in the cerebral tissue after the ischemia. Result: 1) cerebral tissue PAF content was significantly higher in the right hemisphere than in the left hemisphere, respectively(4.40+0.90)and(1.30+0.40)pg/g (P<0.05), after 2-hour reperfusion preceded by 3-hour ligation at the right carotic arteria of gerbils; 2) cerebral tissue PAF content was significantly higher in the right hemisphere than in the left hemisphere, respectively(5.74+0.74)and(0.76+0.05)pg/g (P<0.05), 48 hours after the occlusion at the left middle cerebral artery (MCA) of rats. In the light of the results a analysis was devoted to the possible PAF role in the etiological mechanism of neuron injury coming after cerebral ischemia.