SOCS-1和TNF-α在有机磷中毒致MODS鼠肝脾中表达的研究

Study on the Expression of Socs-1 and TNF-α in Liver and Spleen of Multiple Organ Dysfunction Syndrome Mice Induced by Acute Organophosphorus Pesticide Poisoning (AOPP)

目的探讨SOCS-1和TNF-!在急性有机磷农药(AOPP)致多器官功能障碍综合症(MODS)中的发病机制,从而为MODS的防治提供新的策略。方法健康小鼠随机分成敌敌畏组、生理盐水组、正常对照组,分别于染毒后2、6、12、24h取小鼠的肝脾,以逆转录聚合酶链反应(RT-PCR)测定SOCS-1和TNF-!的mRNA表达水平,用SPSS统计软件进行分析。结果AOPP小鼠肝脾SOCS-1和TNF-!的mRNA水平均升高,与对照组比较差异有统计学意义(P均<0.05)。结论AOPP可导致细胞因子TNF-!失调,可能参与了AOPP后SIRS向MODS的发病过程;TNF-!可诱导SOCS-1在肝脾中表达,考虑到SOCS的"负反馈"调控作用,若能抑制炎症反应的失控,则对中毒MODS的治疗将有新的突破。

Objective To investigate the dynamic of SOCS-1 and TNF-α in liver and spleen of MODS mice induced by AOPP,and discuss the mechanism of MODS induced by AOPP,so that we can give some intervention method over MODS in the future. Method The mice were randomly divided into 2 groups, AOPP group and normal control group. After post poison 2, 6, 12 and 24 hours, liver and spleen tissue were obtained for pathological, mRNA and protein extractions. The expression of SOCS-1 and TNF-α were detected by RT-PCR. The SPSS statistics software was adopted to calculate the correlation. Result The expression levels of SOCS-1 and TNF-α mRNA were significantly increased in the AOPP group when compared with the normal control （P〈0.01）. Conclusion AOPP may up regulate the proinflammatory cytokine, TNF-α, and lead to the development of systemic inflammatory response （SIRS） and hence MODS in AOPP. The up regulation of SOCS-1, a negative regulator of cytokines, may lead to abnormal cytokine signaling. Suppression of the up regulation of SOCS-a and TNF-α may be used as a treatment for AOPP induced MODS.