伪狂犬病毒在潜伏感染猪体内的组织分布

Distribution of Pseudorabies Virus in Tissues of Latently Infected Pigs

潜伏感染是猪伪狂犬病防治和净化工作中的重要障碍之一。本研究用伪狂犬病毒(PRV)gG-/LacZ+标记毒株感染经过PRV灭活疫苗免疫的PRV阴性仔猪,建立了猪伪狂犬病毒潜伏感染动物模型,再用地塞米松激活PRV在猪体内的潜伏感染。运用免疫组织化学SABC染色法研究了PRV在潜伏感染猪和潜伏感染激活猪体内部分组织的分布情况。结果显示,阳性细胞主要分布在神经系统的大脑、小脑、脑干、三叉神经和视神经以及非神经系统的扁桃体、肺和肾等部位。阳性细胞数量随着攻毒后时间的发展呈现减少的趋势,而注射地塞米松后,阳性细胞数量在上述组织中显著增加。

Latent infection is one of the most important obstacles for prevention, control and eradication of pseudorabies in the swine industry. In this study, a pig pseudorabies virus （PRV）-latency model was established by vaccinating PRV-free piglets with commercial PRV inactivated vaccine, followed by challenge with a genetically marked PRV strain gG /LacZ^＋. Then the latency was re-activated by administration of dexamethasone. The distribution of PRV in the tissues of latently infected and re-activated pigs was studied by immunohistochemistry SABC staining with antibodies against β-galactosidase. The results showed that β-galactosidase positive cells were mainly found in the nerve tissues （cerebrum, cerebellum, brain stem, trigeminal nerve and optic nerve） and other tissues （tonsil, lung and kidney）, and the quantities of positive cells decreased along the time post gG-/LacZ^＋ infection. After administration of dexamethasone the numbers of positive cells increased in these tissues.