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胃癌、大肠癌组织MDR1mRNA的定量检测及意义 被引量:6

Significance of MDR1mRNA Quantification in Gastric and Large Bowel Cancers
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摘要 肿瘤的原发与继发性多药耐药已成为临床成功治疗肿瘤的巨大障碍,而这种多药耐药已证明与MDR基因的表达成正相关。根据PCR工作原理设计一种以标准MDR1DNA为参照的PCR定量检测肿瘤组织MDR1mRNA的方法。同时对10例胃癌、6例大肠癌及4例非肿瘤胃组织标本进行了检测。结果有4例MDR1mRNA高表达,其中胃癌3例,大肠癌1例,其余癌组织及非肿瘤胃组织均未检出。3例胃癌均为低分化腺癌,占被检低分化胄腺癌的60%(3/5),大肠癌1例为中分化腺癌。因此,肿瘤组织的MDR1mRNA表达似乎与其组织学类型有关,低分化癌MOR1mRNA呈高表达倾向,这也有可能是低分化癌往往水后化疗不满意的原因之一。 Objective: Is MDR1mRNA measurement useful in planning treatment? Method: According to PCR principle, MDR1mRNA quantification in tumor tissues was performed taking standard MDR1DNA as control. MDR1mRNA of specimens from 10 gastric cancers, 6 large bowel cancers and 4 nonturnorous lesions were measured. The standard MDR1DNA was obtained from recombining MDR1DNA of the blood cell and PGEM-3zf(-) plasmid and was transduced into E Coli.Tissue MDR1cDNA from RT-PCR and standard MDR1DNA were amplified at the same time and with the same primer. The specimen MDR1cDNA was counted through densitometry after electrophoresis which basically represented molecular number of MDR1mRNA. Result: MDR1mRNA increased in 4 cases including 3 gastric cancers and 1 large bowel cancer. All the 3 gastrlc cancers with elevated MDR1mRNA helonged to poorly differentiated adenocarcinoma accountlng 30% of all gastric cancers examined (3/10) and 60% (3/5 ) of all the poorly differentiated gastric adenocarcinomas. The large intestine cancer with elevated MDR1mTNAwas moderatedly differentiated in histology,The result seems that MDR1mRNA expression is related with the histological pattern of the cancer.The poorer the differentiation the higher MDR1mRNA expression is. This may be one reason of unsatisfactory chemotherapy for poorly differentiated cancer.MDR1mRNA quantification has important clinical significance for MDR evaluation.To make comparison before and after chemotherapy and comparison of effects of different drugs might shed lights for reversal of MDR.
出处 《中国肿瘤临床》 CAS CSCD 北大核心 1997年第11期815-818,共4页 Chinese Journal of Clinical Oncology
关键词 胃肿瘤 大肠肿瘤 耐药 MDR1 MRNA 检测 Gastric cancer MDR Large bowel Cancer
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参考文献1

  • 1Shen D W,Science,1986年,232卷,643页

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