VEGF-C局部注射治疗阻塞性淋巴水肿的实验研究

Experimental studies of VEGF-C gene for the treatment of chronic obstructive lymphedema in mouse tail model

目的研究血管内皮生长因子C（VEGF-C）局部注射对治疗小鼠尾部阻塞性淋巴水肿的作用，探讨治疗淋巴水肿的新方法。方法应用RT-PCR、免疫荧光和Western Blot方法检测重组pcDNA3．1-VEGF-C质粒在成纤维细胞和COS细胞中表达和蛋白分泌情况；通过手术方法建立小鼠尾部慢性阻塞性淋巴水肿模型；pCDNA3．1-VEGF-C质粒尾部注射，测量鼠尾直径、鼠尾淋巴管显影、组织学检测及LYVE-1免疫组织化学淋巴管计数，结果进行t检验的统计学分析。结果重组pcDNA3．1-VEGF-C质粒所携带的目的基因人VEGF-C能够在细胞中表达，而且还能够分泌到细胞外；成功建立了小鼠尾部慢性阻塞性淋巴水肿模型；VEGF-C局部注射后，鼠尾直径变小、淋巴管的数目增多，与对照组比较差异有统计学意义（P〈0．05）。结论VEGF-C转基因通过促进淋巴管的再生可以缓解淋巴水肿。

Objective To study the therapeutic effect of vascular endothelial growth factor C（VEGF-C） gene for chronic obstructive lymphedema in mouse tail model which may provide a new treatment for lymphedema. Methods RT-PCR and immunoabsorption were applied to detect VEGF-C gene expression in fibroblasts and secretion of VEGF-C protein in COS7 cells respectively after pCDNA3.1（ ＋ ）VEGF-C transfection .A mouse tail model of chronic obstructive lymphedema was created. Then the pcDNA3.1-VEGF-C plasmid was injected into the tail. The effect of modulating lymphangiogenesis was observed. Results Compared with control group, overexpression of VEGF-C enhanced lymphanglogenesis in vivo and mouse tail skin suffering chronic obstructive lymphedema was improved by VEGF-C gene significantly. Conclusions VEGF-C can improve the lymphedema through enhancing lymphangiogenesis.