摘要
建立了毛细管电泳法分析蘑菇粉中水溶性维生素的方法,并研究缓冲溶液、工作电压等因素对维生素测定的影响。样品进行测定时,先过C18柱,可以对样品中的维生素起到纯化和富集的作用。电泳条件为20kV,在20mmol/LpH8.72硼砂-硼酸缓冲液,10min内实现了蘑菇粉中多种水溶性维生素(VB1、VB2、VB6、VC和VPP)的有效分离,具有良好的稳定性、精密度、线性关系等。
A rapid capillary electrophoresis method was established for detecting water-soluble vitamins in mushroom powder. Effects of buffer solution and voltage on the separation of vitamins from mushroom powder were studied. The effective ingredients of the samples were purified and enriched by means of a solid-phase extraction step with a pre-conditioned C18 cartridge, and the compounds were eluted with pH4.2 HCI solution. VB1, VB2, VB6, VC and VPP could be separated on a 50 cm× 75μm capillary column with 20 mmol/L borate buffer solution （pH8.72）, at temperature 25 ℃ and electrophoresis voltage 20 kV, within 10 rain, and the satisfied results were obtained with different characteristics including linearity, precision and stability.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第5期344-346,共3页
Food Science
基金
安徽省“十五”科技攻关项目(040130042)
关键词
蘑菇粉
高效毛细管电泳
维生素
mushroom powder
Capillary zone electrophoresis
water-soluble vitamins
