宣发膜原法对免疫性关节炎(痹证)小鼠免疫功能的影响

Effect of Immune Function Xuanfa Moyuan Principle on Immune Arthritis (Bizhen) in Rats

目的：探讨宣发膜原法对免疫性关节炎（痹证）小鼠免疫功能的影响。方法：运用Ⅱ型胶原免疫所致小鼠关节炎作为中医痹证动物模型，用过原饮作为宣发膜原法的代表方，对痹证小鼠给药前后关节炎发病程度，血清抗Ⅱ型胶原抗体、ＩｇＭ型类风湿性因子、淋巴结细胞产生白细胞介素１０（ＩＬ－１０）和γ－干扰素（ＩＦＮ－γ）分别用记分法和免疫酶标法进行了检测。结果：宣发膜原法能推迟小鼠关节炎的发病时间，降低发病率，降低小鼠血清抗Ⅱ型胶原抗体ＩｇＧ２ａ、ＩｇＧ２ｂ和ＩｇＧ１水平和ＩｇＧ２ａ＋ＩｇＧ２ｂ与ＩｇＧ１的比值，降低淋巴细胞产生ＩＦＮ－γ的水平，提高其产生ＩＬ－１０的能力。结论：宣发膜原法不仅能抑制小鼠对Ⅱ型胶原的免疫反应，同时能调节小鼠对Ⅱ型胶原的免疫反应。

To explore the effect of Xuanfa Moyuan principle (XFMYP) on arthritic mice. Methods: The type Ⅱ collagen induced arthritis in DBA mice was taken as Bizheng animal model. The Dayuanyin (DYY) was taken as the representative prescription for XFMYP. The arthritis in-cidence and arthritis index were tested by scoring system. The anti-C Ⅱ antibody IgG and its subsets, IgM rheumatoid factor, interferon gamma and interleukin 10 produced by lymph node cells were tested by ELISA method. Results: XFMYP could delay the onset day of arthritis, decrease the arthritic incidence and index, decrease anti-Ⅱ IgG2a, IgG2b,IgG1 level and the IgG2a + IgG2b/IgG1 ratio in serum, decrease the production of interferon gamma by lymphocytes and increase the production of interleukin 10. Conclusions: XFMYP could inhibit the anti-C Ⅱimmune response, and balance the anti-C Ⅱ Th1 and Th2 type immune responses.