蛹虫草菌丝对高血脂调节作用的研究

THE EFFECT OF THE MYCELIA OF CORDYCEPS MILITARIS ON REGULATION OF HIGH BLOOD LIPID

探讨蛹虫草菌丝对高血脂小鼠调节血脂的作用,135只KM小鼠随机分为9组(n=15只/组):正常对照组、高脂模型组、阳性对照组、加热和不加热蛹虫草菌丝液组(低0.14 g/kg.bw/d、中0.28g/kg.bw/d、高0.56 g/kg.bw/d).除正常对照组灌胃等容积的蒸馏水外,其它组均每天早上灌胃高脂乳剂16.5 mL/kg,晚上灌胃给予不同的受试物(33.0 mL/kg),实验持续21 d.每7 d称量小鼠体质量1次,实验第0天、7天、14天剪尾取血检测血清胆固醇(TC),实验第21天摘眼球取血后解剖小鼠,检测血清TC、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C),并计算低密度脂蛋白胆固醇(LDL-C)及肝指数.结果表明:实验第21天,加热和不加热的蛹虫草菌丝液组和金水宝阳性对照组均显著降低高血脂小鼠血清TC和TG水平(P<0.05),并显著降低小鼠肝指数(P<0.01).

The experiment was carried out to investigate the effect of mycelia of Cordyceps militaris on regulating high blood lipid of mice.One hundred and thirty-five KM mice were randomly assigned to nine groups（n=15 in each group）：base control group,high lipid model group,positive control group,the dosage of 0.14、0.28、0.56 g/kg·bw/day mycelia of Cordyceps militaris which was pre-heated or not.While the mice of base control group were received only distilled water of the same cubage by gavage,the others were received high lipid emulsion 16.5 mL/kg in the morning and different samples 33.0 mL/kg in the afternoon by gavage everyday and total for 21 days.The body weigh of mice were weighed every 7 days.The total cholesterol（TC） of serum of the blood which was extracted from the tails was measured on the 0th day、7th day and 14th day.On the 21th day,the mice were dissected after taking out the blood from the mice＇s eyeballs.The TC、triglyeride（TG）、high-density lipoprotein cholesterol（HDL-C） of serum were measured and low-density lipoprotein cholesterol（LDL-C） and liver weigh indices were calculated on the 21th day.The results suggested that the serum TC、TG of high lipid mice which were treated with mycelia of Cordyceps militaris（heated or not）and Jin-shuibao（the positive control group） was significantly decreased on the 21th day（P〈0.05）.The liver weigh indices of the groups treated with mycelia of Cordyceps militaris（heated or not） extremely and significantly decreased（P〈0.01）.