摘要
[目的]优化荷叶提取槲皮素的工艺。[方法]研究了3种提取方法对槲皮素转移率的影响;通过对荷叶中槲皮素的回流提取条件进行4因素3水平正交试验,确定了荷叶中槲皮素的最佳提取工艺。[结果]采用乙醇回流提取法提取荷叶中槲皮素效果最佳,槲皮素转移率均高于60%,明显优于乙醇温浸法(<16.2%)和碱温浸法(<20.0%)。4因素对于槲皮素转移率的影响程度依次为固液比>溶剂浓度>提取时间>提取温度。荷叶中槲皮素最佳提取工艺条件为:乙醇回流提取,乙醇溶液浓度60%,提取温度100℃,料液比12:0,提取时间1 h。[结论]经试验验证,该研究筛选优化的工艺条件基本稳定、可行。在此工艺条件下,槲皮素的转移率在70%以上。
[Objective] This study aimed to optimize extraction technique of quercetin from Lotus leaf.[Method] The effects of 3 kinds of extraction methods on transfer rate of quercetin were investigated. Through the L9 (3^4) orthogonal design experiment about conditions of reflux extraction, the optimum extraction process of quercetin from lotos leaf was ascertained. [Result] The ethanol refluxing method of extracting quercetin from L. leaf could get optimum effect, by which the transfer rate of quercetin was more than 60%. being obviously higher than that by ethanol warm immersion (〈16.2% ) and alkali warm immersion (〈20.0%), resp. The influencing degree of 4 factors on transfer rate in order was ratio of solid to liquid〉 solvent density〉extraetion time 〉 extraction temperature. The optimum process conditions for extracting quercetin from L. leaf were the ethanol refluxing txtraction. 60% ethanol, extraction temperature of 100℃. solid-liquid ratio of 1:20. extraction time of 1 h. [Conclusion] The extraction technique screened by this study was stable and feasible on the whole, under which, the transfer rate of quercetin was over 70%.
出处
《安徽农业科学》
CAS
北大核心
2008年第14期5705-5706,共2页
Journal of Anhui Agricultural Sciences
关键词
荷叶
黄酮
槲皮素
提取工艺
Lotus leaf
Flavonoids
Quercetin
Extraction technique