硫普罗宁对大鼠急性酒精性肝损害的防治作用

Prevention and treatment of acute alcoholically hepatic injury in rats with tiopronin

目的探讨硫普罗宁对大鼠急性酒精性肝损伤动物模型的防治作用。方法健康雄性Wistar大鼠30只,随机分为3组。A组(空白对照组)灌胃生理氯化钠溶液20ml.Kg-1.d-1;B组(模型组)灌胃等剂量白酒;C组(治疗组)在B组基础上预先1h灌胃硫普罗宁100mg.Kg-1.d-1;实验共10天。实验6天、11天取各组大鼠血液,检测谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)、ALT、AST、TG、CHO;实验结束时处死动物,取肝组织,HE、Masson染色,光镜检查。结果在6天和11天时B组大鼠血清ALT,AST,TG和MDA较A组显著升高,GSH-Px降低,C组较B组上述指标均有改善,差异有统计学意义;肝脏病理组织学检查B组织有明显肝细胞脂变和炎症坏死,C组较B组改善,差异均有统计学意义。结论硫普罗宁对大鼠急性酒精性肝损伤有明显的防治作用。

Objective To assess the protective and therapeutic of tiopronin on acute alcoholically hepatic injury in rats. Methods Thirty male Wistar rats were randomly assigned to one of the three groups： Group A （placebo group） was given orally by saline at the dose of 20ml·Kg^-1·d^-1 , the other groups were 56% alcohol at the dose of 20ml·Kg^-1·d^-1. Group C（treatment group） was beforehand administened orally by tiopronin at the dose of 100mg·Kg^-1·d^-1 All rats were treated for 10 days. At the 6th day and the llth day of the treatment, the rat bloods were collected to measure alanine amino transaminase （ALT）, aspartate amino transaminase （AST）, triglycerides （TG）, cholesterol （CHO）, glutathione peroxidase（GSH- Px）, malondialdehyde （MDA）. When the experiment was over, rats were euthanized and the histopathologic alterations were observed. Results At the d6 and dl 1, compared with group A, Group B showed severe hepatic injury with abnormally higher levels of MDA, ALT, AST, TG and the lower GSH - Px, as well as hepatocyte fatty degeneration, even cellular necrosis. Treatment with tiopronin of group C only caused mild abnormalities in the blood tests and hepatic injury. The difference of each groups had statistical sense. Conclusion Tiopronin may prevent rats from acute alcohol- induced hepatic injury.