摘要
应用逆转录聚合酶链反应（ＲＴ＿ＰＣＲ）方法快速克隆人甲状腺过氧化物酶（ｈＴＰＯ）抗原决定簇基因１７０２～２６２２（编号相对于起始密码，编码ＡＡ５６８～ＡＡ８７４）。将此片段重组入ＰＵＣ１８质粒，并用ＰＣＲ＿双链ＤＮＡ（ｄｓＤＮＡ）方法测定全部顺序。结果表明确实克隆到ｈＴＰＯ（１７０２～２６２２）区的基因片段，有两个单碱基。
The human thyroid peroxidase (hTPO) epitopes gene (1702_2622, encoding AA568_AA874) was amplified by RT_PCR and inserted into PUC18 vector for cloning in the present study. The cloned gene was sequenced with dsDNA Cycle Sequencing System. The identity of the cloned hTPO epitopes gene was demonstrated. There were 2 bases different from the cDNA sequence published by Kimura. These changes did not interfere with translation of the protein.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
1997年第3期158-161,共4页
Chinese Journal of Endocrinology and Metabolism
