不同温度焚烧对成人股骨16个基因位点的影响

Diversity of 16 genetic locus in adult femoral bone incinerated at different temperatures

目的：探讨不同温度焚烧后骨骼DNA浓度的变化以及16个基因位点的存留情况。方法：常压下以100-500℃对30例成人股骨进行焚烧，对其存留DNA进行浓度测定，用Identifiler^TMPCR试剂盒进行荧光标记和复合扩增，ABI3100xl Genetic Analyzer软件对DNA16个基因位点进行检测和分析。结果：骨骼样本提取的DNA浓度在50．6～0．0ng／μl之间。未烧和5个温度焚烧后基因位点存留不同，位点检出率分别为98．5％、82．7％、52．9％、35．0％、8．5％、0．4％。结论：温度对骨骼DNA的保存有很大影响，温度越低，保存越完整；片段越小，存留越多。

Objective： To investigate DNA concentration variation and diversity of 16 genetic locus in adult femoral bone incinerated at different temperatures. Methods： 30 samples of the adult femoral bone were incinerated at 100℃, 200℃, 300℃, 400℃ and 500℃ （normal pressure）, and the concentration and purity quotient of DNA were tested following fluorescent labelling and multiplicity amplification by AmpFLSTR IdentifilerTMpCR Kit. 16 genetic locus were analyzed using ABI 3100xl Genetic Analyzer software. Results： The concentration of DNA extraction was at 50.6-0.0 ng/μl. All the 16 genetic locus in the burned bone at five different temperatures were distinct from the unburned bone, and the detection rate of remaining genetic locus was 98.5~, 82.7~, 52.9~, 35.0~, 8. 5~ and 0. 4~. Conclusion ： Temperature played a key role in the preservation of DNA in the adult femoral bone. The lower temperature is benefical to the better integrity of DNA; the smaller fragment can be remained easily.