朗德鹅填饲后不同组织PPARγ基因mRNA表达量差异的初步研究

Pilot Study of the Expression Level of PPARγ Gene in the Different Tissue of Landres Goose

本研究采用荧光定量PCR检测了填饲、未填饲朗德鹅肝脏、皮下脂肪、腹脂和肌肉组织PPARγ基因mR-NA的表达水平,并与谷丙转氨酶等6种血清生化指标进行了相关性分析。结果表明:在肝脏组织中,填饲组PPARγ基因mRNA的表达量极显著高于对照组中该基因的表达量(P<0.01);肝脏组织PPARγ基因mRNA表达水平与肝脏指数、血清谷丙转氨酶含量呈显著正相关(P<0.05),腹脂PPARγ基因mRNA表达水平与肝脏指数、血清谷丙转氨酶含量呈显著正相关(P<0.05),皮下脂肪PPARγ基因mRNA表达水平与肝脏指数、血清谷丙转氨酶含量呈极显著正相关(P<0.01)。结果表明肝脏、皮下脂肪、腹脂组织PPARγ基因mRNA的表达水平与朗德鹅肥肝形成有一定的关系。

In the study, The expression of PPARγ gene in liver, subcutaneous fat, abdominal adipose and muscle were determined by real time PCR, and the relativity between serum parameters and the expression of PPARγ gene in different tissues were analysed. In the overfeeding group the expression of liver PPARγ gene was significantly higher than that of control （P〈0. 01）. In the liver and abdominal adipose, the level of PPARγ gene mRNA are positively related with liver weight/body weight and ALT significantly （P〈0. 05）. In the subcutaneous fat, the level of PPARγ gene mRNA has very significantly correlation coefficient with liver weight/body weight and ALT （P〈0.01）. The result showed that the level of PPARγ gene mRNA in liver, subcutaneous fat and abdominal adipose was related with the forming of Landres goose foie gras.