摘要
目的研究广东凉茶颗粒对拘束负荷诱发小鼠生殖器官过氧化状态的改善作用。方法18h拘束负荷诱发小鼠生殖器官过氧化损伤,分别利用硫代巴比妥酸法测定睾丸和卵巢丙二醛(MDA)水平,HPLC法测定谷胱甘肽(GSH)含量,比色法测定谷胱甘肽过氧化物酶(GSH-Px)和黄嘌呤氧化酶(XOD)活性,Griess化学法测定一氧化氮(NO)含量,荧光酶标仪测定抗氧化能力指数(ORAC)。结果与拘束负荷模型组相比,广东凉茶颗粒可以明显降低因拘束负荷引起的小鼠睾丸和卵巢组织MDA水平、NO含量和XOD活性升高,同时显著升高睾丸和卵巢组织中的GSH含量、GSH-Px活性和ORAC指数。结论广东凉茶颗粒对拘束负荷诱发的小鼠生殖器官过氧化状态具有一定的改善作用,其作用可能与减少拘束负荷小鼠的氧化应激水平和改善组织脂质过氧化过程有关。
Objective To observe the improvement of Guangdong Liangcha Granules （GLG） on restraint - stress - induced peroxidation in genital organs of mice. Methods Mice models ofperoxidation injury in genital organs were induced by 18 - hour restraint stress. Testicular and ovarian malondialdehyde （MDA） level was detected by thiobarbituric acid method, glutathione （GSH） content by HPLC, xanthine oxidase （XOD） and GSH - PX activities by colorimetric method, nitric oxide （NO） content by Griess chemical method and oxygen radical absorbance capacity （ORAC） by enzyme -linked fluorescent immunoassay. Resuits Compared with model group, GLG can markedly reduce MDA level, XOD acitivity and NO contents, in addition, GLG can effectively increase the ORAC value, GSH content, GSH - PX activity in testis and ovaries. Conclusion Oral treatment of Guangdong Liangcha Granules was found to reduce the status of peroxidation in testis and ovaries, and the improvement may be related to the increase of its free radical scavenging activity and lipid peroxidation.
出处
《中药新药与临床药理》
CAS
CSCD
2008年第4期253-256,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
国家科技支撑计划(2006BAIO6A20-09)
