铝胁迫下大豆根系分泌有机酸和氨基酸的特性

The secretive characteristic of organic acids and amino acids under aluminium stress

采用水培法,以2个大豆(Glycinemax)品种(浙春2号和浙春3号)为材料,研究铝胁迫下大豆根系分泌有机酸和氨基酸的特征。大豆根系分泌大量柠檬酸呈现低铝促进高铝抑制的现象,同时还分泌少量草酸和琥珀酸,表明大豆根系分泌有机酸与其耐铝机制有关。氨基酸分泌总量随着铝质量浓度的增大而增加。中低铝质量浓度下,大豆根系分泌的氨基酸随铝质量浓度的增大而增加。高铝浓度下,氨基酸的种类减少,各种氨基酸分泌量变化不一致。实验结果还显示,随着铝处理时间延长,铝毒害作用明显。

A solution culture experiment was conducted to study the effect of aluminum stress on secretion characteristics of organic acids ands amino acids in two soybean seedlings. The citric acid secretion was induced under low Al3＋ concentration but inhibited under high Al3＋ concentration, and small quantity of oxalic acid and succinic acid exudated, indicating organic acid exudation was involved in the Al-tolerance. Total amino acids secretion increased with the increasing of Al3＋ concentration. More amino acids were secreted under low and middle Al3＋ concentration, but less kinds of amino acids exudated under high Al3＋ concentration, and the content of each amino acids had no obvious rules under high concentration. The experimental results also indicated that the poison effect of aluminum on soybean was more remarkable with the prolonging of AI treatment time.