高效液相色谱定量分析腐乳中的莫纳可林K

Determination of Monacolin K in Sufu by High Performance Liquid Chromatography

采用Nova-PakC18色谱柱(150×3.9mm,4μm),水(含0.3%H3PO4);乙醇为流动相,梯度洗脱,流速0.50ml/min,柱温50℃,测定波长(λ)237nm,定量分析腐乳中莫纳可林K,结果显示,酸型和内酯型莫纳可林K分别在0.2～14mg/L时呈线性关系,加样平均回收率分别为89.7%、94.8%,相对标准偏差1.5%～2.9%。

The monacolin K in sufu was separated by high performance liquid chromatography （HPLC） with Nova-Pak C18 column（150×3.9 mm, 4μm） at 50 ℃.The mixed solution of water （containing 0.3% phosphatic acid） and ethanol was used as mobile phase for gradient elution at a rate 0.50 ml/min. And the absorbance was measured at 237 nm. The results showed that when the contents of acid form and lactone form monacolin k are in 0.2～14 mg/L, there are good linear relationships between absorbance and content respectively. The average recoveries are 89.7%, and 94.8%, and relative standard deviations range between 1.5% to 2.9%, respectively.