大黄、何首乌中大黄素和大黄酚的提取与含量测定

Determination of Emodin and Chrysophanol Contents in Rheum officinale Baill. and Poly gonummultiflorum Thunb.

目的确定大黄、何首乌中大黄素与大黄酚的含量。方法甲醇回流方法提取大黄素与大黄酚,高效液相色谱方法测定大黄素与大黄酚含量。结果大黄中大黄酚的含量是何首乌的83倍,何首乌中大黄素的含量是大黄的3倍。结论这为大黄与何首乌药材的质量控制,制药企业的合理选料及研究大黄素、大黄酚在不同植物中的分布提供技术参数和理论指导。

Objective To determine the emodin and chrysophanol contents in Rheum officinale Baill. and Polygonum multiflorum Thunb, , Methods The emodin and chrysophanol were extracted by means of methanol - reflux, the contents of emodin and chrysophanol were determined by HPLC method, Results The emodin content in Rheum officinale Baill, was 83 times of that in Polygonum multiflorum Thunb,, The chrysophanol content in Polygonum multiflorum Thunb. was three times of that in Rheum officinale Baill, Conclusion This may provide technical parameters and guidance for quality control of Rheum officinale Baill, and Polygonum multiflorum Thunb , rational selecting materials for enterprises, and studying the distribution of emodin and chrysophanol in various plants,