摘要
目的:探讨RU486对离体培养人早孕绒毛滋养细胞分泌PAs及PAI┐1、PAI┐2的影响,比较药物流产与正常绒毛中tPA、uPA、PAI┐1及uPA┐R表达的差异。方法:应用纤维蛋白琼脂糖铺盖及反向铺盖技术分别检测细胞培养液中PA与PAI┐1的活性,采用琼脂糖指示胶直接点样法测定PAI┐2活性。分别应用免疫组织化学与原位杂交技术检测绒毛组织细胞中tPA、uPA、PAI┐1与uPA┐R的抗原及mRNA的存在与分布。结果:受RU486刺激,离体培养绒毛滋养细胞分泌的PA┐PAI复合物的水解活性增加,而PAI┐1与PAI┐2的活性则受抑制。口服米非司酮药流绒毛滋养细胞中PAI┐1及uPA抗原含量低,而uPA┐R的mRNA含量显著增加。结论:RU486增加早孕绒毛中纤溶活性,从而使细胞外基质蛋白水解作用加强,绒毛的固着性可能因此松动。
Objective:To investigate the effects of RU486(mifepristone) on release of PAs and PAI1,PAI2 in cultured trophoblastic cells,and on expression of tPA,uPA,PAI1,uPAR in human early placental villi.Methods:SDSPAGE followed by fibrin overlaid and reverse overlaid techniques were used to determine PAs and PAI1 activities respectively in media for culture of trophoblastic cells.PAI2 activities from media were measured by PAI indicating agarose gel directely.The immunohistochemical and in situ hybridization methods were used to detect the antigens and mRNAs of tPA,uPA,PAI1 and uPA-R in early villi from women requesting termination of pregnancy of 6 ̄8w by curettage or drugabortion with RU486.Results:In vitro cultured trophoblastic cells secreted PAPAI complex and PAI1 and PAI2.RU486 increased fibrinolytic activity of PAPAI complexes and decreased activities of PAI1 and PAI2.Cytoand syncytiotrophoblastic cells in early villi contained antigens and mRNAs of tPA,uPA,PAI1 and uPAR.The antigen contents of PAI1 and uPA were lower in both trophoblastic cells from villi treated by RU486 than that in the normal one.While RU486 highly increased the mRNA of uPAR in the both cells.Conclusions:RU486 increases the activity of PAPAI complex,but decreases the activities of PAI1 and PAI2 in cultured trophoblastic cells in vitro,RU486 reduces PAI1,but increses mRNA of uPAR in both trophoblastic cells of villi in vivo.These effects of RU486 can activate the fibrinolytic processes in extracellular proteins to induce contraearlygestation.
出处
《现代妇产科进展》
CSCD
1997年第4期307-311,316,共6页
Progress in Obstetrics and Gynecology
基金
国家自然科学基金
计划生育生殖生物学国家重点实验室基金