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RU486对人早孕绒毛中纤维蛋白溶酶原激活与抑制系统的影响 被引量:5

Influence of RU486 on the activating and inhibiting system of plasminogen in human early pregnancy villi
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摘要 目的:探讨RU486对离体培养人早孕绒毛滋养细胞分泌PAs及PAI┐1、PAI┐2的影响,比较药物流产与正常绒毛中tPA、uPA、PAI┐1及uPA┐R表达的差异。方法:应用纤维蛋白琼脂糖铺盖及反向铺盖技术分别检测细胞培养液中PA与PAI┐1的活性,采用琼脂糖指示胶直接点样法测定PAI┐2活性。分别应用免疫组织化学与原位杂交技术检测绒毛组织细胞中tPA、uPA、PAI┐1与uPA┐R的抗原及mRNA的存在与分布。结果:受RU486刺激,离体培养绒毛滋养细胞分泌的PA┐PAI复合物的水解活性增加,而PAI┐1与PAI┐2的活性则受抑制。口服米非司酮药流绒毛滋养细胞中PAI┐1及uPA抗原含量低,而uPA┐R的mRNA含量显著增加。结论:RU486增加早孕绒毛中纤溶活性,从而使细胞外基质蛋白水解作用加强,绒毛的固着性可能因此松动。 Objective:To investigate the effects of RU486(mifepristone) on release of PAs and PAI1,PAI2 in cultured trophoblastic cells,and on expression of tPA,uPA,PAI1,uPAR in human early placental villi.Methods:SDSPAGE followed by fibrin overlaid and reverse overlaid techniques were used to determine PAs and PAI1 activities respectively in media for culture of trophoblastic cells.PAI2 activities from media were measured by PAI indicating agarose gel directely.The immunohistochemical and in situ hybridization methods were used to detect the antigens and mRNAs of tPA,uPA,PAI1 and uPA-R in early villi from women requesting termination of pregnancy of 6 ̄8w by curettage or drugabortion with RU486.Results:In vitro cultured trophoblastic cells secreted PAPAI complex and PAI1 and PAI2.RU486 increased fibrinolytic activity of PAPAI complexes and decreased activities of PAI1 and PAI2.Cytoand syncytiotrophoblastic cells in early villi contained antigens and mRNAs of tPA,uPA,PAI1 and uPAR.The antigen contents of PAI1 and uPA were lower in both trophoblastic cells from villi treated by RU486 than that in the normal one.While RU486 highly increased the mRNA of uPAR in the both cells.Conclusions:RU486 increases the activity of PAPAI complex,but decreases the activities of PAI1 and PAI2 in cultured trophoblastic cells in vitro,RU486 reduces PAI1,but increses mRNA of uPAR in both trophoblastic cells of villi in vivo.These effects of RU486 can activate the fibrinolytic processes in extracellular proteins to induce contraearlygestation.
出处 《现代妇产科进展》 CSCD 1997年第4期307-311,316,共6页 Progress in Obstetrics and Gynecology
基金 国家自然科学基金 计划生育生殖生物学国家重点实验室基金
关键词 米非司酮 中断妊娠 早孕绒毛 RU486 PA-PAI系统 Human early pregnancy villi Plasminogen activators and inhibitors RU486(mifepristone)
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