姜黄素对缺氧条件下HepG2细胞VEGF表达的影响

Effect of curcumin on vascular endothelial growth factor expression in HepG2 hepatocellular carcinoma cells under hypoxic conditions

目的:研究姜黄素对缺氧条件下人肝癌细胞HepG2中血管内皮生长因子(VEGF)表达的影响.方法:姜黄素(0、1、2、5、10μmol/L)处理缺氧条件下的HepG2细胞6h后,采用四唑盐(MTT)法观察HepG2细胞活性的变化,Westernblot观察VEGF的蛋白水平变化,RT-PCR检测VEGF mRNA表达水平变化.结果:HepG2细胞经1、2、5、10μmol/L的姜黄素处理后,VEGF蛋白和mRNA水平与对照组(0μmol/L姜黄素处理)比较明显降低(蛋白:2.12±0.23,1.59±0.13,0.82±0.11,0.33±0.05vs2.85±0.37,P<0.05或P<0.01;mRNA:0.60±0.05,0.54±0.04,0.16±0.02,0.06±0.01vs0.81±0.07,均P<0.01),且降低程度随着姜黄素处理浓度的增加而变大.结论:姜黄素可抑制HepG2细胞中VEGF的基因表达.

AIM： To investigate the effect of curcumin on the expression of vascular endothelial growth factor （VEGF） in hepatocellular carcinoma cell line HepG2 under hypoxic conditions. METHODS： Hepatocellular carcinoma HepG2 cells were treated with different concentra- tions （0, 1, 2, 5, 10μmol/L） of curcumin under hypoxic conditions for 6 h. Cellular viability was detected by MTT assay.The level of VEGF expression was detected at protein and mRNA level by Western blot technique and reverse transcription-poly chain reaction respectively. RESULTS： The levels of VEGF protein and mRNA decreased significantly in the cells co-incu- bated with curcumin at 1, 2, 5, and 10 μmol/L for 6 h in a dose-dependent manner as compared with those in the control cells （0 μmol/L curcumin treatment） （VEGF protein： 2.12 ± 0.23,1.59 ± 0.13, 0.82 ± 0.11, 0.33 ± 0.05 vs 2.85 ± 0.37, P 〈 0.05 or P 〈 0,01; VEGF mRNA： 0.60 ± 0.05, 0.54 ± 0.04, 0.16 ± 0.02, 0.06 ± 0.01 vs 0.81 ± 0.07, all P 〈 0.01）. CONCLUSION： Curcumin can decrease the expression of VEGF in hepatocellular carcinoma HepG2 cells.