王老吉凉茶对应激小鼠糖代谢机能及体内过氧化状态的影响

Effect of Wanglaoji Cool Tea on plasma gluco metabolism and peroxidative state in stress mice

目的:研究王老吉凉茶(岗梅根,木蝴蝶,火炭母,金钱草,布渣叶,淡竹叶,金沙藤,五指柑,山芝麻,金樱根)对拘束负荷诱发小鼠糖代谢机能低下及体内过氧化状态的影响。方法:将雄性BALB/c小鼠分为正常对照组、应激对照组、阳性对照组、125及500 mg/kg王老吉凉茶给药组,每天灌胃给药一次连续给药5 d后拘束负荷应激20 h,灌胃葡萄糖2mg/kg检测血糖消除能力、血浆酮体水平、肝糖原合成能力、血浆过氧化状态及抗氧化能力水平。结果:与应激对照组相比,王老吉凉茶给药组能提高应激小鼠血糖消除速率,降低血浆酮体水平并促进肝糖原合成能力。此外,王老吉凉茶明显改善拘束负荷升高的血浆脂质过氧化反应的产物丙二醛(MDA)含量,提高血浆抗氧化能力指数(ORAC)水平。结论:王老吉凉茶改善拘束负荷诱发小鼠糖代谢机能低下,其作用机制可能与缓解机体内的过氧化状态有关。

AIM： To study the effect of Wanglaoji cool tea（WLJ） （Ilex asprella,Oroxylum indicum,polygnum chinese, Desmadium Styracifolium , Microcos Paniculata , Lophatherum gracile , Lygodium japonicum , Vitex negundo , Helicteres angustifolia,Rosa laevigate） on plasma gluco-metabolism and peroxidative state in stress mice. METHODS： The male BALB/c mice were randomly divided into normal control group, stress control group, positive control group, 125 and 500 mg/kg WLJ group, which were administered samples once a day successively for 5 days. After oral administration 2 g/kg glucose into the 20 h stress mice, the elimination rate of plasma glucose, plasma ketone bodies and liver glycogen were determined. The peroxidative state in plasma and antioxidtive capacity of plasma was also measured. RESULTS： As compared with the control groups, WLJ accelerated the basic glucose metabolism of plasma, reduced the plasma ketone body and elevated the liver glycogen synthesis in stress mice. And it also decreased the level of MDA and increased the antioxidant capacity of plasma. CONCLUSION： WLJ improves the glucometabolic dysfunction in stress mice, and the mechanism might be related to the amelioration of peroxidative state in plasma.