人类趋化素样因子超家族在非小细胞肺癌中的表达

Expression of CKLF1, CMTM1, CMTM2 and CMTM4 in non-small cell lung cancer

目的探讨人类趋化素样因子超家族（CKLFSF）成员CKLF1、CMTM1、CMTM2和CMTM4在非小细胞肺癌（NSCLC）组织中的表达及其临床意义。方法应用组织微阵列和免疫组织化学方法检测50例NSCLC组织和癌旁正常肺组织中CKLF1、CMTM1、CMTM2和CMTM4蛋白的表达及差异。结果CKLF1、CMTM1、CMTM2和CMTM4蛋白在癌旁正常肺组织中的阳性表达率均为100％，在NSCLC组织中的阳性表达率分别为12％、2％、14％和26％，差异有统计学意义（P〈0．01）。性别、病理分化程度、病理分期与CKLF1、CMTM1、CMTM2和CMTM4蛋白在NSCLC组织中的表达程度之间无明显相关（P〉0．05）。肺腺癌组织中CMTM1、CMTM4蛋白的表达明显高于其在鳞癌组织中的表达（PcmTm1=0．011，PcmTm4〈0．01）。结论位于人类染色体同一基因簇内的趋化素样因子超家族成员CKLF1、CMTM1、CMTM2和CMTM4可能是潜在的NSCLC抑癌基因。

Objective To investigate the expression of CKLF1 ,CMTM1 ,CMTM2 and CMTM4 in non-small-cell lung cancer （NSCLC）. Methods The expression of CKLF1, MTM1, CMTM2 and CMTM4 proteins in 50 cases of NSCLC and corresponding adjacent normal lung tissues was detected by tissue microarray and immunohistochemistry, Results The positive expression rate of CKLF1, CMTM1, CMTM2 and CMTM4 was all 100% in the adjacent normal lung tissues,and 12% ,2% , 14% ,26% respectively in NSCLC tissues （P 〈 0.01 ）, The expression of CKLF1, CMTM1, CMTM2 and CMTM4 had no correlation with the gender,pathological differentiation and stage （P 〉 0.05）, The expression of CMTM1 and CMTM4 in the adenocarcinomas was higher than in the squamous cell carcinomas （ Pcm Tm1 = 0.011, Pcm Tm4 〈 0. 01）. Conclusion CKLF1 ,CMTM1 ,CMTM2 and CMTM4 were potential antioncogenes.