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表皮生长因子对猕猴胚胎角膜上皮组织增殖效应的研究 被引量:1

THE EFFECT OF MOUSE EPIDERMAL GROWTH FACTOR ON CELL PROLIFERATION OF CORNEAL EPITHELIUM OF MONKEY EMBRYONIC EYES
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摘要 离体培养试验表明,小鼠颌下腺表皮生长因子对离体培养猕猴胚胎眼角膜上皮组织具有显著促进细胞增殖和分化的效应。培养4d的实验组角膜上皮的厚度,上皮细胞增殖层数以及上皮组织中基底层细胞核与表层细胞核两者的比率均较正常猴胚角膜增长3倍。与此同时培养4d的实验组角膜上皮厚度和上皮细胞的层数,都达到成年猴正常角膜上皮的结构水平。 The corneal epithelium of monkey (Macaca mulatta) embryonic eyes has been cultured in vitro. Results indicated that there was an obvious effect of cells proliferation and differentiation on the comeal epithelium of Macaca mulatta embryonic eyes induced by epidermal growth factor (EGF) of mouse submaxillary gland. The thickness of comeal epithelium, the number of cells layer of the epithelium and the ratio between the nuclei number of basement layer cells and the nuclei number of surface layer cells were all increased three times in EGF groups than that of the control gtoups(without EGF added) in vitro culture four days.
出处 《江西科学》 1997年第3期149-153,共5页 Jiangxi Science
关键词 表皮生长因子 猴胚胎 角膜上皮 多肽 增殖效应 Epidermal growth factor(EGF), Monkey embryo, Comeal epithelium
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  • 1黄平,石星源,童坦君.表皮生长因子对正常细胞与转化细胞染色质蛋白激酶活性的影响[J].生物化学杂志,1989,5(6):503-508. 被引量:4
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  • 3Yadwad VB,Meghji K,Ward OP.Rapid purification and partial characterizstion of recombinant human epidermal growth factor produced by Escherichia coli-protei purification by solid-phase extraction and HPIC.Biotechnol Tech,1993,7(9):657.
  • 4Haryae N,Ishiyama A,Niwano M.Secration of human epidermal growth factor in autotropic culture by a recombinant hydrogen-utilizing bacterium,Pseudomonas paeudoflave,carrying brosd-host-range EGF secretion veotor PKSEGF 2-protein secration in Pseudomonas aeruginoss,Pseudomonas putida and expression with carbon dioxidefixation in Alcaligenes eutrophus,Alcaligenes paradoxus and Paracoccus denitrificans.with carbon dioxidefixation in Alcaligenes eutrophus,Alcaligenes paradoxus and Paracoccus denitrificans.Appl Environ Micrbiol,1994,60(9):3336.
  • 5郑子修,李靖炎,钟金颜.小鼠表皮生长因子对鸡胚角膜上皮组织的增殖效应[J].江西科学,1997,15(2):77-80. 被引量:1

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