柴郁温胆汤对大鼠抑郁模型行为学及脑内单胺类神经递质的影响

Effects of Chaiyuwendan Decoction on ethology and monoamine neurotransmitters in brain of depressive disorder rats

目的:探讨中药复方柴郁温胆汤抗抑郁作用的机制。方法:以孤养加慢性轻度不可预见性应激方法建立大鼠抑郁症模型,用旷场行为测定及糖水消耗试验进行行为学评分,并用高效液相-电化学方法检测其脑内单胺类神经递质的含量,观察模型大鼠给药前后的变化。结果:抑郁症模型大鼠体质量增长缓慢,水平和垂直活动及糖水消耗量均明显减少(P<0.05,P<0.01);杏仁核NE、DA、5-HT、HVA含量减少(P<0.05);纹状体NE、DA、5-HT含量减少(P<0.05)。柴郁温胆汤能改善模型大鼠行为学变化,升高杏仁核NE、5-HIAA、5-HT、HVA(P<0.05)及DA含量(P<0.01),升高纹状体DA和5-HT含量(P<0.05)。结论:柴郁温胆汤具有抗抑郁作用,对中枢单胺类神经递质的调节作用是其疗效机制之一。

Objective： To study the anti-depression mechanism of Chaiyuwendan Decoction （CYWDD）. Methods： Rat models were established by separation and chronic unpredictable mild stress （CUMS）. Ethology of rats were detected by open-field test and sucrose consumption test, contents of monoamine were detected by HPLC-ECD.Resnlts Compared with normal group, the weight, the frequency of crossing, rearing and the contents of consumption to sucrosum water in depressive disorder rats decreased significantly （P〈0.05, P〈0.01）, the contents of NE, DA, 5-HT and HVA in amygdaloid nucleus decreased significantly （P〈0.05）,and the contents of NE, DA and 5-HT in corpus striatum of depressive disorder rats decreased significantly （P〈0.05）.CYWDD can improve ethology of depression rats and increase the contents of NE, 5-HIAA, 5-HT and HVA in amygdaloid nucleus significantly （P〈0.05） and increase the contents of DA significantly （P〈0.01）in the amygdaloid nucleus. In the corpus striatum, CYWDD can increase the contents of DA, 5-HT significantly （P〈0.05）. Conclusion： The effects of anti-depression of CYWDD may be related to the regulation of monoamine neurotransmitters in brain.