川渝地区花生品种遗传多样性分析

Analysis of genetic diversity in cultivated peanut(Arachis hypogaea L.) germplasms from Sichuan & Chongqing

将AFLP标记与表型性状相结合,分析了来自川渝地区的两个亚种四个类型的43份主要花生种质资源和推广品种的遗传多样性。结果表明,两种方法都能有效地检测到该生态区内花生种质间的遗传多样性,珍珠豆型内和龙生型内品种间距离较小,而普通型内和中间型内品种间距离较大;交替开花亚种的龙生型和普通型品系间距离最近,而珍珠豆型与其它三个类型间距离较远;主要推广品种间遗传多样性匮乏。聚类结果也基本一致,都能将珍珠豆型与其他三大类型区分开,而龙生型、普通型和中间型在聚类中都有交叉情况。但在较为细小的类群划分上,两种方法仍有较大差异。

The genetic diversity of 43 breeding lines and released cultivars representing four botanical varieties of two subspecies were assessed by clustering analysis based on AFLP markers and eight agronomic traits. The genetic distance within var. vulgarls and var. hirsuta were smaller, and the distance were larger within var. hypogaea and the improved breeding lines derived from crosses involving genotypes belonging to different subspecies as parents. The distance between var. hirsuta and var. hypogaea was the closest, while the distance between var. vulgaris and the other three varieties was the greatest. The genetic variation detected among the major cuhivars were very limited. It is necessary to exploit native germplasm and introduce exotic germplasm in order to improve peanut in Siehuan ＆ Chongqing region. The results of clustering based on AFLP markers and phenotypie traits mostly were consistent. The lines from var. vulgaris were clustered in a group, and could be separated from the other three varieties. However, there was greater difference in clustering smaller group between these two methods.