NK细胞活性与血清可溶性CD25水平检测在继发性噬血细胞性淋巴组织细胞增多症诊断中的意义

Diagnostic Significance of NK Cell Activity and Soluble CD25 Level in Serum from Patients with Secondary Hemophagocytic Lymphohistiocytosis

本研究探讨继发性噬血细胞性淋巴组织细胞增多症(secondary hemophagocytic lymphohistiocytosis,HLH)患者外周血NK细胞活性、血清可溶性CD25(sCD25)水平的检测在早期诊断中的意义。收集2005年6月至2008年5月继发性HLH疑似病例38例,25名正常健康人员作为对照,采用LDH释放法检测外周血NK细胞活性,ELISA法检测血sCD25的水平,38例疑似病例根据HLH-2004诊断标准分为排除组和诊断组,比较各组NK细胞活性及sCD25水平的差异,同时比较确诊组确诊前后各项诊断标准的符合情况。结果表明:38例疑似患者中有22例最终确诊为继发性HLH,其NK细胞活性均明显低于正常对照组,差异有显著统计学意义(p<0.001),血清sCD25水平明显高于正常对照组,差异亦有统计学意义(p<0.05),且确诊组患者的NK细胞活性和血清sCD25水平在疾病早期100%出现异常。结论:NK细胞活性及血清sCD25水平的检测对于继发性HLH的早期诊断可能具有重要的意义。

This study was aimed to explore the clinical significance of NK cell activity and serum soluble CD25 （ sCD25 ） level in early diagnosis of the patients with secondary hernophagocytic lymphohistiocytosis （HLH）. 38 suspected secondary HLH patients from June 2005 to June 2008 and 25 healthy subjects were enrolled in the study. The NK cell activity in peripheral blood was determined by a released LDH assay, The sCD25 level in serum was detected with ELISA double antibody sandwich assay. The 38 suspected secondary HLH patients were divided into diagnosed and excluded group according to HLH-2004 diagnostic criteria, The NK cell activity and sCD25 level were compared between the two groups. The results showed that 22 out of 38 suspected patients were diagnosed as secondary HLH, the NK cell activity in peripheral blood of these 22 patients was significantly lower than that of healthy control （p 〈 0. 001 ）, the sCD25 level in peripheral blood of these 22 diagnosed patients was higher than that of healthy control （p 〈0.05）. In conclusion, detection of NK cell activity and sCD25 level may be valuable in the early diagnosis of secondary HLH.