TACE蛋白在动情周期和围着床期小鼠子宫内膜的表达规律

Expression Pattern of TACE Protein in Mouse Endometrium during the Estrus Cycle and the Peri-implantation Period

目的:初步探讨肿瘤坏死因子转换酶(TACE)蛋白在动情周期和早孕小鼠子宫内膜的表达规律。方法:用免疫组化SP法检测动情周期和早孕小鼠子宫内膜TACE蛋白的表达。结果:TACE蛋白在各期小鼠子宫内膜呈规律性表达,动情周期中动情期与其它3期相比表达明显增强(P<0.001),妊娠期比动情期表达显著增强(P<0.001),孕d3、d4、d5、d6与孕d1、d2、d7相比表达显著增强(P<0.001)。结论:TACE可能通过裂解多种细胞表面分子促进子宫内膜同步发育、胚胎粘着、侵袭等过程,在胚泡植入中发挥着重要作用。

Objective： To investigate the expression pattern of tumor necrosis factor - converting enzyme （TACE） in mouse endometrium during the estrus cycle and early pregnancy. Methods： Immunohistochemistry SP method was used to detect the expression of TACE protein in mouse endometrium during the estrns cycle and early pregnancy. Results： The expression of TACE protein was detected in mouse endometrium at different stages regularly. Compared with the other 3 stages, the level of TACE protein expression at estrus was obviously higher （P 〈 0.001 ）. The intensity of TACE protein expression during pregnancy was stronger than that of the estrus cycle （P 〈0. 001 ） , and the level of TACE protein expression was higher at d3, d4, d5, and d6, compared with the d1 , d2, and d7 of pregnancy （P 〈0. 001 ）. Conclusion： TACE may play important roles in blastocyst implantation with its proteolysis of several cell surface molecules and its promotion of these processes such as endometrium synchronism, embryo adhesion, and invasion.