维生素E缺乏对雏鸡红细胞膜磷脂及流动性的影响

Effect of vitamin E deficiency on the phospholipid and liquidity of RBC membrane in chicken

用低维生素E和添加不饱和脂肪酸的日粮饲喂雏鸡,建立VE缺乏实验动物模型,采用荧光偏振法检测红细胞膜流动性,高效薄层层析法检测红细胞膜组分。结果表明,VE缺乏组雏鸡红细胞膜磷脂酰胆碱(PC)、磷脂酰乙醇胺(PE)含量降低,神经鞘磷脂(SM)、磷脂酰丝氨酸(PS)含量升高,膜流动性下降,组间差异显著或极显著(P<0.05,P<0.01)。提示VE对维持雏鸡红细胞膜结构和功能起重要作用。

The model chicken with the vitamin E （VE）deficiency was set up by feeding low VE diet and adding unsaturated fatty acid in the feed. The content of phospholipid and fluidity of RBC membrane were detected by fluorescence polarization and high performance thin-layer chromatography（HPTLC）, respectively. The results showed that the content of phospholipids PC and PE,and fluidity in RBC membrane in VE deficiency chicken were lower than that of control group;the content of SM and PS were higher than that of control group（P〈0.05 ,P〈0.01）. The results indicated that VE plays an important role in maintaining structure and function of RBC membrane in chicken.