外周血Th1/Th2细胞在大鼠肝移植早期的变化

Changes of Th1/Th2 in peripheral blood in early stage of allogeneic orthotopic rat liver transplantation

目的观察外周血Th1/Th2细胞在大鼠肝移植早期不同免疫状态下的变化趋势是否和免疫状态有关。方法大鼠分3组:A组:同基因移植组(BN→BN);B组:异基因移植(Lewis→BN)+环孢素A(CsA)组;C组:异基因移植组。均36只,另外各设观察组观察生存期。于移植后1、3、5、7和14d应用流式细胞分析外周血CD4+CD45RC±的百分数,观察移植肝排斥反应病理分级及受体存活时间。结果(1)A组和B组的生存期平均大于100d,明显长于C组(34.3±3.6)d。(2)除第1天外,C组和B组各时间段的排斥分级明显高于A组(P<0.05或<0.01);C组亦明显高于B组(P<0.05或<0.01)。(3)移植后3d起CD4+CD45RC+%/CD4+CD45RC-%在C组明显高于A组和B组(P<0.05或<0.01)。(4)CD4+CD45RC+%/CD4+CD45RC-%在B组和排斥分级存在负相关(r=-0.565,P<0.01),而在C组CD4+CD45RC+%/CD4+CD45RC-%和排斥分级存在正相关(r=0.745,P<0.01)。结论外周血Th1/Th2细胞的变化趋势和大鼠肝移植早期不同免疫状态有关。

Objective To investigate whether the tendency of Th1/Th2 cell in peripheral blood is associatied with different immune states in early stage of allogeneic orthotopic rat liver transplantation. Methods Allografted rats were divided to three groups（ each group n = 36）： syngeneic control（ group A）, the group of allograft treated with CsA 2 mg/（ kg.d）（ group B） and allograft （group C）. At post-transplantation day of 1,3,5,7,14, six rats from each group were sacrificed to harvest allografted liver for pathological, percentage of CD4^＋ CD45RC^± T cell subsets in peripheral blood of recipient by flow cytometry, and additional subgroups （ n = 6） for observation of survival time. Results （ 1 ） The survival time of both group A and group B were significantly longer than that of group C （ 〉 100 day vs 34. 3 ±3.6 days, P 〈0. 01 ）. （2） Apart from 1 day, the rejection grades were higher in the group C and B than that in group A and there were significant differences between the two groups （P 〈 0. 05 or 〈 0.01 ）. （3） From third day to fourteenth day post - transplantation , the ratio of CD 4^＋~ CD 4 5 RC^＋ percentage with CD 4^＋ CD45RC^- percentage was significant higher in group C than that in group B and group A （ P 〈 0. 05 or 0. 01 ）. （4）There was negative correlation between ratio of CD4^＋ CD45RC^＋%/CD4^＋ CD45RC^-% and rejection grades （ r = -0. 565 ,P 〈 0. 01 ）, but positive correlation existed between ratio of CD4^＋ CD45RC^＋ %/CD4^＋ CD45RC^- % and rejection grades（ r = 0. 745 ,P 〈 0. 01 ）. Conclusion The ratio of Th1/Th2 cell in peripheral blood varied according to different immune states in early stage of allogeneic orthotopic rat liver transplantation.