离子凝聚法制备负载流感疫苗的壳聚糖微球

Preparation of Influenzal Vaccine Chitosan Microparticles by Ionic Gelation Method

采用三聚磷酸钠(TPP)作为离子交联剂,应用离子凝聚法制备负载流感疫苗的壳聚糖微球.筛选出壳聚糖起始质量分数为1%.TPP的浓度对壳聚糖微球的制备影响较大,采用低浓度的TPP(200μg/mL)制备的微球放置过夜均出现沉淀现象,高浓度的TPP(800μg/mL)在制备过程中出现絮状沉淀.固化比影响微球的释放行为,固化比为1∶1的微球爆炸式释放率达到90%,固化比为1∶3的微球6 h后逐步释放,12 h后释放率达到95%.固化比为1∶5的微球6 h后没有明显的释放行为.壳聚糖溶液的pH对微球的制备和释放没有显著的影响.通过对负载流感疫苗的壳聚糖微球的制备条件和释放行为的研究结果表明,pH=5.6的壳聚糖溶液,固化比为1∶3,TPP的质量浓度为400μg/mL是较理想的流感疫苗壳聚糖微球的制备条件.

Influenzal vaccine chitosan microparticles were prepared with tripolyphosphate （TPP） by ionic gelation method. The working mass fraction of chitosan was 1%. Morphologies of chitosan microparticles were affected by concentration of TPP, lower concentration of TPP （200 μg/mL） made the microparticle solution give sediment overnight, Whereas, higher concentration of TPP（800 μg/mL） made microparticles gather to gether. The release of vaccine from chitosan microparticles decreased when cross-linking ratio increased. The pH of chitosan solution had no effect on morphologies and release behavior. The results indicate that pH = 5.6, cross-linking ratio 1 ： 3, mass concentration of TPP 400 μg/mL are the optimum conditions to prepare influenzal vaccine chitosan microparticles via morphologies and release behavior.