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经气管／静脉途径行肺脏腺病毒载体基因转染的效率和毒性分析 被引量：3

The effectiveness and toxicity of recombinant adenovirus vector transferation via trachea and vein
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摘要 目的:分别经气管和静脉途径转染腺病毒载体基因至肺组织,探索不同给药途径对肺组织的转染效率和腺病毒载体转基因治疗的安全性。方法:分别通过气管内滴入和静脉注射途径向家兔转染携带绿色荧光蛋白(GFP)基因的腺病毒载体(Ad-GFP 2×109pfu),于第3 d、7 d、14 d、21 d处死动物,荧光显微镜下观察测肺、肝脏、心脏组织GFP表达,HE染色观察组织病理改变。结果:气管转染组第3 d仅在肺组织内表达GFP,持续21d,且强度大;静脉注射转染组第3 d可在肺、肝脏、心脏组织测及GFP表达,14 d皆消失,强度较弱。HE染色显示,气管转染组第3 d可见肺脏炎性反应,第14 d炎性反应消失,肝脏、心脏均未见炎性反应;静脉注射转染组第3 d可见肺脏、心脏及肝脏内有轻度炎性反应,7 d后炎性反应皆消失。结论:肺脏经气管途径转染腺病毒载体具有较高的靶向性和较长时间基因表达的优点,虽可造成肺部一过性免疫炎性反应,仍不失为肺脏转基因治疗的有效和相对安全的途径。
作者 王伟 吴树明 张中明 张宜乾
机构地区 徐州医学院附属医院心胸外科 山东大学齐鲁医院心脏外科
出处 《中国病理生理杂志》 CAS CSCD 北大核心 2008年第12期2493-2496,共4页 Chinese Journal of Pathophysiology
基金 国家自然科学基金资助项目(No.30571845) 江苏省自然科学基金资助项目(No.BK2007033) 江苏省高校自然科学基金资助项目(No.07KJD310218)
关键词 腺病毒载体 绿色荧光蛋白 基因 转染 Adenovirus vector Green fluorescent protein Gene Transfection
分类号 R363 [医药卫生—病理学]
  • 相关文献

参考文献12

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共引文献3

同被引文献19

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引证文献3

二级引证文献8

中国病理生理杂志
2008年 第12期

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