Runx3与Ki67分子在慢性胃炎胃黏膜肠化生及胃癌组织中的表达及相互关系

Expression of Runx3 and Ki67 in chronic gastritis with intestinal metaplasia and gastric carcinoma and their correlations

目的:探讨人类runt相关转录因子3(Runx3)和Ki67分子在慢性胃炎胃黏膜肠化生和胃癌发生、发展中的作用及二者的相互关系,分析两者在胃黏膜肠化生和癌变过程中的作用.方法:采用免疫组化SP法对92例正常胃黏膜、胃黏膜肠化生及胃癌标本进行Runx3和Ki67染色.分析Runx3和Ki67在慢性胃炎胃黏膜肠化生和胃癌中的表达,以及与胃癌组织学类型和生物学行为关系,并分析Runx3和Ki67表达的相互关系.结果:Runx3在胃黏膜肠化生和胃癌组织的表达明显低于正常胃黏膜(26.7%,10.6%vs100%,均P<0.01).而Ki67分子在胃黏膜肠化生与胃癌中的表达率明显高于正常胃黏膜(40%,74.5%vs 2%,均P<0.01).Runx3的表达与胃癌的分化程度、浆膜浸润、淋巴结转移有关(χ2=7.729,7.661,4.656,P=0.021,0.006,0.031),Ki67的表达与分化程度、浆膜浸润、胃癌组织学类型有关(χ2=6.758,4.019,9.740,P=0.034,0.045,0.002).Runx3和Ki67在胃黏膜肠化生和胃癌中表达呈显著负相关(r=-0.453,P<0.01).结论:检测Runx3和Ki67表达可能有助于胃癌高危人群,尤其是慢性胃炎患者伴有肠化生-胃癌演变过程的人群的筛查、早期诊断.

AIM：To examine the expression and roles of Runx3 and Ki67 in the process of the intestinal metaplasia as well as their roles in gastric carcinogenesis.METHODS：Immunohistochemical staining was used to examine the expression and cor-relation of Runx3 and Ki67 in 92 cases of gastric specimens,including 15 cases of normal gastric mucosa,30 cases of intestinal metaplasia and 47 cases of gastric cancer.The relationship between their expressions and histological types or bio-logical behavior was analyzed.RESULTS：Runx3 was expressed in all normal gastric mucosa.Compared with normal gas-tric mucosa a significantly lower expression was found in chronic gastritis with intestinal metaplasia and in gastric cancer（26.7%,10.6% vs 100%,both P〈0.01）.The expression rate of Ki67 was higher in gastric cancer than in chronic gastritis with intestinal metaplasia（40%,74.5% vs 2%,both P〈0.01）.In gastric carcinoma,the expression of Runx3 was related to differential degree of tumor,serosal infiltration and metas-tasis to lymph nodes（χ2=7.729,7.661,4.656,P= 0.021,0.006,0.031）.The expression of Ki67 was related to differential degree of tumor,serosal infiltration and histological types（χ2=6.758,4.019,9.740,P=0.034,0.045,0.002）.There was a negative correlation between the Runx3 expres-sion and Ki67 expression in chronic gastritis with intestinal metaplasia or in gastric cancer（r =-0.453,P〈0.01）.CONCLUSION：Runx3 and Ki67 might be uti-lized jointly to screen for high-risk population and early diagnosis of gastric cancer.