费尔干猪毛菜愈伤组织的诱导、分化及植株再生体系的建立

Callus Induction,Differentiation and Establishment of Plantlet Regeneration System of Salsola ferganica

探讨了植物生长调节剂、外植体等因子对费尔干猪毛菜(Salsola ferganicaDrob.)愈伤组织诱导、分化与植株再生的影响。结果表明:2,4-D与6-BA组合使用时,在一定浓度范围内均有愈伤组织产生,最佳的诱导培养基为MS+2,4-D 2.0 mg/L+6-BA0.2 mg/L,下胚轴为诱导愈伤组织的最佳材料;愈伤组织继代培养较好的培养基为MS+NAA0.1 mg/L+6-BA1.0 mg/L;愈伤组织不定芽分化培养基为MS+6-BA 0.5 mg/L+NAA 0.05 mg/L;根分化的培养基为1/2 MS+IBA0.2 mg/L,且生根率可达72%。以带柄子叶为外植体,诱导丛生芽的最佳分化培养基为MS+6-BA1.0 mg/L,再生频率可达90.74%。

The effects of explants and plant growth regulators on callus induction, differentiation and plantlet regeneration of Salsola ferganica Drob. were studied. The results showed that the best medium of callus induction was MS medium with 2,4-D 2.0 mg/L ＋6-BA 0. 2 mg/L. Hypocotyl was the best explant. The best medium for the subculture of callus was MS ＋ NAA 0. 1 mg/L ＋ 6-BA 1.0 mg/L. The MS medium with 6-BA 0.5 mg/L ＋ NAA 0. 05 mg/L was preferable for inducing adventitious shoot. The 1/2 MS medium with IBA 0. 2 mg/L was suitable for the root differentiation and the rate of rooting was 72%. The best medium for inducing adventitious shoot of cotyledon with petiole was MS ＋ 6-BA 1.0 mg/L and the shoot regeneration frequency was 90. 74%.