不同麻醉剂量灌喂对南方鲇幼鱼代谢的影响

Effect of Force-feeding on Postprandial Metabolism in Anaesthetized Juvenile Southern Catfish with Different Doses

绝大多数的鱼类餐后代谢即特殊动力作用(SDA)研究都是以实验条件下能够主动摄食的鱼类为对象,近年来麻醉灌喂方法越来越多地用于不主动摄食鱼类的相关研究,而目前专门评价麻醉灌喂方法的文献资料却不多见。本研究为了考察麻醉灌喂对南方鲇(Silurus meridionalisChen)幼鱼SDA的影响,采用4、7、10g/L的不同剂量麻醉剂间氨基苯甲酸乙酯甲磺酸盐(MS-222)处理,以主动摄食组为对照,均灌喂10%南方鲇幼鱼体重的泥鳅(Misgurus anguillicanndotus),连续48h(1次/2h)测定各组实验鱼的耗氧率。结果显示,各实验组的SDA参数之间,除10g/L麻醉灌喂组的摄食代谢峰值比率与对照组存在显著差异外(p<0.05),其它参数如静止代谢率、SDA时间、峰值达到时间、摄食代谢峰值、SDA总能量和SDA系数各实验组之间均无显著差异(p>0.05)。上述结果表明,麻醉和灌喂操作对南方鲇幼鱼的SDA过程无明显影响,该方法不仅适用于南方鲇摄食代谢的研究,还将有助于获取不主动摄食鱼类的相关资料。

Those fishes which can take food initiatively in experiment have been studied in many fish researches of postprandial metabolism, namely specific dynamic action （SDA）. Recently the method of force-feeding following anaesthesia has been used in more and more relative researches into those fishes which can＇t take food initiatively. However, little literature specially has valued this method at present. To investigate the effect of force-feeding on SDA in anaesthetized juvenile southern catfish, anaesthetized with different doses （4, 7, 10 g/L） and subsequently force-fed by a same level （10 % body mass） forage （loach, Misgurus anguillicanndotus） in the present experiment. Meanwhile, initiative-feeding group is compared. Then the rates of oxygen consumption in each group are measured for 48 h （once per two hours）. The results reveal that there are no significant differences among each group in almost every SDA variables （resting metabolic rate, SDA duration, time to peak, peak Fo2 , energy expended on SDA and SDA coefficient included） （p 〉 0.05）, except that there is significantly different between 10 g/L anaesthetized force-feeding group and initiative-feeding group in factorial metabolic scope （p 〈0.05 ）. The above results suggest that anaesthesia and force-feeding have no significant effect on SDA procedure in southern catfish. This method is not only fit to developing feeding metabolism research in southem catfish, but also helpful to gain relative data in those fish which can＇ t take food initiatively.