FMDV OA／58病毒株VP3蛋白结构的模拟与分析

Construction and Analysis of VP3 Protein from A Foot-and-mouth Disease Virus Strain OA/58

以口蹄疫病毒株OA／58 RNA为模板，反转录并扩增目的cDNA，然后与pMD18-T载体连接并转化JM109菌株，提取的重组质粒用凝胶电泳、PCR和BarnHⅠ、HindⅢ双酶切法鉴定。分析表明，口蹄疫病毒VP1、VP2、VP3和VP4在核苷酸水平上的变异率是无差异的（P〉0．05）；而它们在氨基酸水平上的变异率差异显著（P〈0．05）。该毒株与20株源于GenBank中的VP3氨基酸序列比较发现其保守区主要位于第1～24、26～35、37～43、45～57、61～122、124～173、175～209、211～220位。运用同源模建，OA／58 VP3蛋白三维空间结构可分为A，B，C3个结构区域。保守区氨基酸残基在维持VP3蛋白的空间构象和功能方面具有重要作用。

Foot-and-mouth disease virus strain OA/58 RNAs were used as templates for RT-PCR. The amplified cDNA products were cloned into pMD18-T vectors and transformed into JM109. The recombinant plasmids were identified by electrophoresis, PCR, and analysis of tow cleavages with BamH Ⅰ and Hind Ⅲ. After analyzing the difference among VP1, VP2, VP3, VP4, at the nueleotide level, the mutation rates of these 4 encoding sequenees were no difference （p 〉 0.05）, however, at the amino acid level, those mutation rates were different （p 〈 0.05）. The regions of 1 - 24th, 26 - 35th, 37- 43th, 45 -57th, 61 - 122th, 124- 173th, 175- 209th and 211 -220th in VP3 protein most probably are conservative. Depending on homology modeling the FMDV strain OA/58 VP3 protein, the 3D mold was gained. And this 3D mold was divided into A, B and C regions. This research should be used as an instruetion in order to direct the work on FMDV VP3 protein.