摘要
Three kinds of autotrophic bioleaching bacteria strains,including mesophilic and acidophilic ferrous ion-oxidizing bacteria Acidithiobacillus ferrooxidans(A.ferrooxidans),mesophilic and acidophilic sulfur-oxidizing bacteria Acidithiobacillus thiooxidans(A.thiooxidans),and moderately thermophilic sulfur-oxidizing bacteria.4cidianus brierleyi,were cryopreserved in liquid nitrogen and their ferrous ion-or sulfur-oxidizing activities were investigated and compared with the original ones.The results revealed that ferrous ion/sulfur oxidation activities of the strains were almost equal before and after cryopreservation.Glycerin was used as cryoprotective agent.In conclusion,liquid-nitrogen cryopreservation is a simple and effective method for autotrophic bioleaching microorganisms.
Three kinds of autotrophic bioleaching bacteria strains, including mesophilic and acidophilic ferrous ion-oxidizing bacteria Acidithiobacillus ferrooxidans （A. ferrooxidans）, mesophilic and acidophilic sulfur-oxidizing bacteria Acidithiobacillus thiooxidans （A. thiooxidans）, and moderately thermophilic sulfur-oxidizing bacteria Acidianus brierleyi, were cryopreserved in liquid nitrogen and their ferrous ion- or sulfur-oxidizing activities were investigated and compared with the original ones. The results revealed that ferrous ion/sulfur oxidation activities of the strains were almost equal before and after cryopreservation. Glycerin was used as cryoprotective agent. In conclusion, liquid-nitrogen cryopreservation is a simple and effective method for autotrophic bioleaching microorganisms.
出处
《中国有色金属学会会刊：英文版》
CSCD
2008年第6期1386-1391,共6页
Transactions of Nonferrous Metals Society of China
基金
Project(50621063)supported by Chinese Science Foundation for Distinguished Group
Project(2004CB619201)supported by the National Basic Research Program of China
关键词
液态氮
超低温保存
生长活性
细菌
A. ferrooxidans
A. thiooxidans
Acidianus brierleyi
cryopreservation
liquid nitrogen
growth activities
