Real-time PCR和PCR方法快速检测犬细小病毒

Rapid detection of canine parvovirus by Real-time PCR and PCR methods

为适应出入境口岸对进出境宠物快速检疫的需要,本研究在建立PCR方法检测犬细小病毒(CPV)的基础上,进一步采用Taqman探针技术建立了快速检测CPV的Real-time PCR方法。通过灵敏度对比试验,证实Real-time PCR方法比PCR方法检测灵敏度显著提高。通过对大量不同采样部位样品的检测证实,本研究建立的Real-time PCR和PCR方法具有较高的可靠性,并可显著提高CPV的阳性检出率。

Canine parvovirus disease caused by canine parvovirus （CPV） is one of the most important canine diseases with widespread infection and high mortality, which is one of the crucial quarantine diseases for entry-exit pets. In order to accelerate inspection efficiency,the methods of PCR and Real time PCR using TaqMan probe were established. Comparing with PCR method, Real-time PCR assay was proved to be specific with high sensitivity and efficiency. Further,a great quantity of CPV positive samples collected .from different sites were detected by Real-time PCR and PCR to evaluate the reliability of these motheds. The results show Real-time PCR and PCR methods have high reliablity and significantly improve the detection efficiency of CPV positive samples.