VIP在胃腺癌中的表达及临床意义的研究

Vasoactive intestinal peptide expression and its clinical significance in gastric adenocarcinoma

目的研究血管活性肠肽（VIP）在胃腺癌组织和血浆中的表达及其与临床病理因素的关系。方法采用ELISA法检测胃腺癌患者血浆中和正常对照组的VIP水平，RT-PCR方法检测VIP mRNA在胃腺癌组织、其相邻的正常胃腺癌组织及不典型增生胃黏膜的差异表达量，Western Blot法进一步检测胃腺癌组织中VIP蛋白的表达。结果ELISA法检测正常人血浆VIP水平为（5．794±0．014）ng／ml，胃腺癌患者血浆中的VIP水平为（14．437±0．825）ng／ml，两者差异有统计学意义（P〈0．05）。RT-PCR分析胃腺癌组织中VIP mRNA的V／B值明显高于不典型增生胃黏膜及相应的癌旁正常胃黏膜，其V／B值分别为：（1．5261±0．3028）、（0．9334±0．2872）、（0．90514-0．2794），差异有统计学意义（P〈0．01）；正常胃窦黏膜与不典型增生胃黏膜中，V／B值的差异无统计学意义（P〉0．05）；不同分化程度的胃腺癌组织VIP mRNA表达量的差异有统计学意义（P〈0．05）；有淋巴结转移与无淋巴结转移的胃腺癌组织VIP mRNA表达量之间差异亦有统计学意义（P〈0．05）。Western Blot法检测胃腺癌组织VIP蛋白表达量高于正常组织。结论VIP可能参与了胃腺癌的发生。VIP蛋白的过度表达可能与胃腺癌的分化程度、侵袭有关，其表达对判断预后有参考价值。

Objective To investigate the expression of vasoactive intestinal peptide （VIP）in gastric adenocarcinoma, and to evaluate the correlation of VIP level with clinical pathologic parameters. Methods The level of VIP in sera from gastric adenocarcinoma patients and healthy people was investigated by ELISA. Moreover, the differential gene expression between gastric adenocarcinoma, gastric dysplasia, and the corresponding normal gastric mucosa were determined by RT-PCR. Western Blot was also used to measure the expression of VIP in the gastric adenocarcinoma and the normal gastric mucosa. Results The serum level of VIP was （5.794±0.014）ng/ ml in normal control and was （14.437±0.825） ng/ml in gastric adenocarcinoma patients, showing significant difference （P 〈 0.05）. Meanwhile, the V/B of gastric adenocarcinoma tissues was greater than that of gastric dysplasia and the corresponding normal gastric mucosa （P〈0.01）,the values of V/B were 1.5261± 0.3028, 0.9334±0.2872, and 0.9051±0. 2794, respectively. The values of V/B between normal gastric mucosa and gastric dysplasia were not different significantly （P〉0.05）. There were significantly negative correlation between the VIP mRNA expression of the differentiation degree of tumor（P〈0.05）. The VIP mRNA expression was higher in gastric adenocarcinoma with lymph node metastasis than that without lymph node matastsis （P〈0.05）. The VIP protein expression of the gastric adenocarcinoma tissues was greater than that of normal control. Conclusion This findings provide a direct evidence to support the possibility that VIP play a cofactor role in the pathogenesis of gastric adenocarcinoma.