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利用RAPD-PCR检测三种白僵菌及球孢白僵菌种内变异 被引量:22

DETECTION OF VARIATION BETWEEN 3 SPECIES OF BEAUVERIA AND ISOLATES OF BEAUVERIA BASSIANA BY RAPD-PCR TECHNIQUES
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摘要 采用PCR-PAPD技术研究了球孢白僵菌2株野生型多孢株及其各1株单孢分离株的RAPD扩增片段的多态性,比较了彼此间的相似率。结果显示,相似率在63.6%~99.3%之间变化很大,表明各分离子间的基因组DNA已发生变化。球孢白僵菌与另一种白僵菌布氏白僵菌之间的相似率在64.9%~80.9%之间,与粘孢白僵菌之间的相似率在50.0%~61. In the present paper, PCR-RAPD techniques were used to study the polymorphism of RAPD-amplified fragments between saltation segregants and 2 wild type multi-spored strains of Beauveria bassiana and their mutual similarities were compared.The results showed that the similarities varied within 63.6%~99.3%, suggesting obvious change at genome DNA level of the segregants. The similarities between the isolates of B. bassiana and an isolate of B. brongniartii were 64.9%~80.9%, while those between the isolates of B. bassiana and an isolate of B. velata were 50.0%~61.9%.
出处 《菌物系统》 CAS CSCD 北大核心 1998年第2期185-189,共5页 Mycosystema
基金 国家自然科学基金 中国科学院真菌地衣系统学开放实验室资助
关键词 球包白僵菌 布氏白僵菌 PCR RAPD 多态性 eauveria bassiana, Beauveria brongniartii, Beauveria velata, PCR,RAPD, Polymorphism
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