期刊文献+

广西水牛瘤胃中的细菌多样性 被引量:6

Bacterial diversity in Guangxi buffalo rumen
原文传递
导出
摘要 【目的】了解广西水牛瘤胃中细菌的组成及其可能的降解纤维素细菌的主要类群。【方法】提取水牛瘤胃内容物和高效降解滤纸的水牛瘤胃内容物的富集培养物的宏基因组DNA,以宏基因组DNA为模板,扩增16S rRNA基因序列,构建该两种样品的细菌的16S rRNA基因文库。通过对16S rRNA基因序列的分析,了解这两种样品的细菌群体种类及数量。【结果】水牛瘤胃内容物与其富集培养物中均主要含有LGCGPB(low G+C Gram-Positive Bacteria)、CFB(Cytophaga-Flexibacter-Bacteroides)两大类菌群和少数的螺旋体菌(Spirochaetes),且LGCGPB所占的比例都是最高的,LGCGPB在水牛瘤胃内容物细菌中的比例为56.66%,而在富集培养物细菌中的比例升高为73.33%。在水牛瘤胃内容物中,丝状杆菌(Fibrobacteres)占3.33%,但在富集培养物中未被检测到。而在富集培养物中占13.33%的变形杆菌(Proteobacteria),在水牛瘤胃内容物中未被检测到。本研究还发现了分类地位尚未明确的一菌群(R46)。【结论】细菌类群LGCGPB、Proteobacteria可能在水牛瘤胃中的纤维素降解过程中起重要作用。此外,水牛瘤胃中的细菌组成和牦牛、牛、羊瘤胃中的细菌组成较相似但比例有所不同。 [Objective]To analyze the diversity of bacterial community in Guangxi buffalo rumen and to identify the possible cellulolytic bacterial group. [Methods] Metagenomic DNAs were isolated directly from a buffalo rumen and its enriched culture,and were used as PCR templates to amplify 16S rRNA genes. Two libraries carrying 16S rRNA genes of bacteria in the two samples were constructed. The bacterial community composition was revealed by the constructed phylogenetic tree of known sequences and the sequences randomly selected from the libraries. [Results] We found that both samples contained low G+C Gram-positive bacteria (LGCGPB) and Cytophaga-Flexibacter-Bacteroides (CFB) phyla as the majorities,and Spirochaetes as the minorities. LGCGPB accounts for 56.66% and 73.33% of the bacterial communities in buffalo rumen and its enriched culture. We detected Fibrobacteres in the rumen sample (3.33%) but not in the enriched sample. Furthermore,we found Proteobacteria as a major component in the enrichment (13.33%) but not in the rumen sample. Clone R46 was not clustered into any known phyla and might belong to a novel taxonomic group. [Conclusion] The LGCGPB and Proteobacteria may play important roles in the hydrolysis of cellulose in buffalo rumen. The bacterial composition in the rumen of buffalo is quite similar to those in the rumen of yak,cattle and sheep.
出处 《微生物学报》 CAS CSCD 北大核心 2009年第2期251-256,共6页 Acta Microbiologica Sinica
基金 国家自然科学基金(30560003) 广西亚热带生物资源保护利用重点实验室开放课题(SB0708)~~
关键词 瘤胃细菌 16S RRNA基因 细菌多样性 rumen 16S rRNA gene bacterial diversity
  • 引文网络
  • 相关文献

参考文献13

  • 1Woese CR. Bacterial evolution. Microbiological Reviews, 1987,51:221 - 271.
  • 2Tajama K. Rumen bacterial diversity as determined by sequence analysis of 16S rDNA libraries. FEMS Microbiology Ecology, 1999,29 : 159 - 169.
  • 3石鹏君,柏映国,袁铁铮,姚斌,范云六,周志刚,孟昆,伍宁丰,刁其玉.应用rpoB和16S rDNA基因的变性梯度凝胶电泳技术对山羊瘤胃细菌多样性的研究[J].微生物学报,2007,47(2):285-289. 被引量:22
  • 4王远亮,杨瑞红,毛爱军,王加启,董志扬.采用未培养技术对荷斯坦奶牛瘤胃细菌多样性进行初步分析[J].微生物学报,2005,45(6):915-919. 被引量:25
  • 5An DD, Dong XZ, Dong ZY. Prokaryte diversity in the tureen of yak (Bos grunniens ) and Jinnan Cattle (Bos taurus ) estimated by 16S rDNA homology analyses. Anaerobe, 2005,11 : 207 - 215
  • 6Garcia-Martinez DV, Shinmyo A, Madiu A, et al. Studies on cellulase production by Clostridium thermocellum. European Journal Applied Microbiology Biotechnology , 1980,9:189 - 197.
  • 7Zhou J, Bruns MA, Tiedje JM. DNA recovery from soils of diverse composition. Appl Environ Microbiol, 1996,62 ( 2 ) : 316 - 322.
  • 8Feng Y, Duan C J, Pang H, et al. Cloning and identification of novel cellulase genes from uncultured microorganisms in rabbit cecum and characterization of the expressed cellulases. Appl Microbiol Biotechnol, 2007,75 : 319 - 328.
  • 9Lane DJ. 16S/23S rRNA sequencing. In: Stackebrandt and M. Goodfellow (ed.), Nucleic acid techniques in bacterial systematics. United Kingdom: John Wiley & Son, Chichester, 1991 ,pp115 - 175.
  • 10Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology Evolution, 1987,4:406 - 4251.

二级参考文献38

  • 1姚文,朱伟云,韩正康,Antoon D L Akkermans,Barbara Williams,Seerp Tamminga.应用变性梯度凝胶电泳和16SrDNA序列分析对山羊瘤胃细菌多样性的研究[J].中国农业科学,2004,37(9):1374-1378. 被引量:53
  • 2Akin D E, Borneman W S. Role of rumen fungi in fiber degradation. J Dairy Sci, 1990, 73: 3023-3032.
  • 3Hungate R. The rumen and its microbes. New York: Academic Press, 1966.
  • 4Raizada N, Sonakya V, Dalhoff R, et al. Population dynamics of rumen microbes using modern techniques in rumen enhanced solid incubation. Water Sci Technol, 2003, 48(4):113-119.
  • 5Eun J S, Fellner V, Gumpertz M L. Methane production by mixed ruminal cultures incubated in dual-flow fermentors. J Dairy Sci, 2004, 87(1):112-121.
  • 6Roderick I, Bryan A. Recent advances in rumen microbial ecology and metabolism: potential impact on nutrient output. J Dairy Sci, 1990, 73: 2971-2995.
  • 7Barnes S P, Keller J. Anaerobic rumen SBR for degradation of cellulosic material. Water Sci Technol, 2004, 50(10):305-311.
  • 8Hungate R, Bryant M P, Mah R A. The rumen bacteria and protozoa. J Annu Rev Microbial, 1964, 18: 131-166.
  • 9Bryant M P. Bacterial species of the rumen. J Bacteria Rev, 1959, 23:125-153.
  • 10Caldwell D R, Bryant M P. Medium without rumen fluid for nonselective enumeration and isolation of rumen bacteria.J Appl Microbiol, 1966, 14(5): 794-801.

共引文献42

同被引文献134

引证文献6

二级引证文献61

;
使用帮助 返回顶部