羟基喜树碱联合奥沙利铂对人肺癌细胞作用的体外研究

In vitro interactions between hydroxycamptothecine and oxaliplatin on human lung carcinoma cell line

目的:体外观察羟基喜树碱(hydroxycamptothecine,HCPT)联合奥沙利铂(oxaliplatin,L-OHP)对人肺癌A549细胞增殖的协同抑制作用,并初步探讨其机制。方法:采用SRB法测定HCPT和L-OHP对A549细胞增殖的抑制作用,根据中效原理判断两药合用时的效应。采用显微镜、流式细胞仪观察两药对A549细胞的诱导凋亡作用。结果:HCPT和L-OHP均能明显抑制A549细胞增殖和诱导细胞凋亡,两药合用具有协同作用。结论:HCPT联合L-OHP能协同抑制A549细胞增殖,其机制可能是通过协同诱导凋亡。

Objective：To study the combined effect of hydroxycamptothecine（HCPT） and oxaliplatin（ L- OHP） on human lung adenocarcinoma A549 cells in vitro, and investigate the possible mechanism. Methods： Cell growth inhibition was assesed by Sulforhodamine - B technique. Median - effect analysis was used to determine interactions between HCPT and L - OHP. Apoptotic cells was detected by fluorescence microscope and flow eytometry. Results： HCPT and L - OHP given alone or in combination could inhibit the growth of A549 cells effectively. Combination treatment had synergistic cytotoxicity on A549 cells. Both HCPT and L - OHP could induce the apoptosis of A549 cells, and apoptotic rate was obviously elevated by combination treatment. Conclusion： HCPT and L - OHP in combination showed synergistic cytotoxicity on A549 cells. Combination treatment induced more apoptosis of A549 cells, it maybe involved in the mechanism of the synergistic cytotoxicity.