慢性应激对小鼠空间学习记忆功能及海马和前额叶皮层BDNF表达的影响

Effects of Chronic Stress on Ability of Spatial Learning-Memory and Expression of Brain Derived Neurotrophic Factor in The Hippocampus and Prefrontal Cortex of Mice

采用多因素慢性应激动物模型,通过Morris水迷宫测试小鼠空间学习记忆能力;采用免疫组织化学方法检测脑源性神经营养因子(BDNF)在海马和前额叶皮层的表达.结果显示,与对照组相比,应激组小鼠的空间学习记忆能力明显下降(P<0.01);应激组小鼠海马CA1区、齿状回和前额叶皮层BDNF表达明显下降(P<0.01);停止应激后一周,应激组小鼠BDNF在各脑区的表达有一定恢复,但与对照组相比,仍有显著性差异(P<0.05,P<0.01).结果表明,慢性应激导致小鼠空间学习记忆功能的损伤可能与BDNF表达的下调密切相关.

This paper mainly stnded study the effects of chronic stress on the ability of spatial learning and memory of mice using Morris water maze, and the expression of brain derived neurotrophie factor （BDNF） in the hippocampus and prefrontal cortex （PFC） by immunohistochemical method. The results showed that compared with control group, the ability of spatial learning and memory were significantly decreased （P 〈0.01 ） ; and the BDNF expression in the CA1 region and dentate gyms of hippocampus and PFC were significantly reduced in stress group mice （P 〈0. 05, P 〈0.01） on after stress and 7 days after stress period. The results indicate that chronic stress causes the damage of mice＇s spatial learning and memory function which may be nearly related to the down-regulation of BDNF expression.