摘要
目的分析浙江省2006-2007年暴发性病毒性胃肠炎中诺如病毒感染的分子流行病学特征。方法收集监测期间暴发性病毒性胃肠炎疫情患者的粪便标本及相关流行病学资料。采用RT-PCR及荧光定量RT-PCR检测诺如病毒。随机选择部分阳性标本扩增部分多聚酶区和衣壳蛋白片段，进行序列测定，结合诺如病毒I（GI）、Ⅱ（GⅡ）基因型参考株进行进化分析。结果诺如病毒感染暴发性胃肠炎共5起，发生时间均集中于9-12月，送检标本63份，诺如病毒检测阳性45份。序列分析结果显示，浙江省诺如病毒序列与诺如病毒GⅡ／4型参考株同源性最高，其中与北京2006年、荷兰2006年诺如病毒GⅡ／4型变异株最为接近，核苷酸同源性分别为99.7％和98.5％-99.0％。诺如病毒与北京、荷兰流行的GⅡ／4型变异株处于同一分支。结论诺如病毒是浙江省病毒性腹泻暴发疫情的重要病原体，流行时间集中于秋季，其流行株为GⅡ／4型变异病毒株。
Objective To study the molecular epidemiological characteristics of Norovirus gastroenteritis outbreaks in Zhejiang. Methods During January 2006 and December 2007, fecal specimens of patients collected from outbreaks of acute viral gastroenteritis were tested for Norovirus. Epidemiological data were also collected. Noroviruses were detected by a reverse transcription polymerase chain reaction （RT-PCR） and Real-time RT-PCR. Some positive samples were randomly selected and RT-PCR products were sequenced. Comparing to the nucleotide sequences of norovirus genotype Ⅰ,Ⅱ reference strains from GenBank, sequence analysis was undertaken based on partial sequence of RNA dependent RNA polymerase （RdRp） and capsid protein （VP1） gene. Results 5 outbreaks of viral gastroenteritis caused by Norovirus were reported. A total of 63 stools were obtained from cases with acute gastroenteritis. Noroviruses alone were detected in 45 cases and the illness appeared in autumn. Phylogenetic analysis revealed that Norovirus belonged to G Ⅱ/G Ⅱ 4 type. The strains isolated from Zhejiang were almost identical on G Ⅱ/4 variants that causing epidemics in Beijing and in the Netherlands with the homology of 99.7% and 98.5%-99.0% respectively. Phylogenetic analysis revealed that the isolates were located at the same branch as the norovirus G Ⅱ/4 variants found in Beijing and Netherlands. Conclusion Norovirus is a major cause of outbreaks of viral gastroenterifis in Zhejiang province.Genogroup Ⅱ/4 variants viruses were the prevalent strains.
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2009年第2期147-150,共4页
Chinese Journal of Epidemiology
