摘要
目的:观察黄连解毒汤对小鼠脑缺血慢性神经损伤的保护作用。方法:以大脑中动脉阻塞(MCAO)15min诱导小鼠短暂性局灶性脑缺血。从术前7d开始,用药组灌服黄连解毒汤2g/kg和4g/kg,实验对照组和假手术组灌服生理盐水,均连续给药21d,一日一次。缺血后35d(5周)内进行神经症状评分、斜板试验,并记录小鼠的最终生存率。实验结束后,测定脑损伤体积和神经元数量。结果:黄连解毒汤4g/kg能显著提高小鼠MCAO术后35d的最终生存率,2g/kg和4g/kg均明显改善术后的神经症状,降低脑梗死体积、减轻脑萎缩。黄连解毒汤4g/kg可明显增加缺血侧海马CA1区、纹状体和皮层的神经元密度,2g/kg明显增加缺血侧海马CA1区的神经元密度。结论:黄连解毒汤对小鼠局灶性脑缺血慢性神经损伤有保护作用,能提高小鼠的最终生存率并促进神经功能的恢复。
Objective: To investigate the neuroprotective effects of Chinese herb medicine Huanglian-diedu-Tang (HJDT) on chronic brain injury after focal cerebral ischemia in mice. Methods: Focal cerebral ischemia was induced by occlusion of right middle cerebral artery (MCA) for 15 rain. HJDT (at dosage of 2 g/kg or 4 g/kg,qd,orally) was administered for 21 d from d 7 before isehemia until d 14 after ischemia. The sham and ischemic controls were administered with normal saline orally. The neurological deficit scoring and the inclined board testing were performed within 35 d after ischemia. The survival rate,the infarct volume and the neuron density were assessed 35 d after ischemia. Results: HJDT increased the survival rate at dose of 4 g/kg; significantly reduced the neurological deficits,infarct volume and cerebral atrophy at doses of 2 and 4 g/kg after ischemia; and significantly increased the neuron density in the
出处
《浙江大学学报(医学版)》
CAS
CSCD
北大核心
2009年第1期75-80,共6页
Journal of Zhejiang University(Medical Sciences)
基金
浙江省中医药管理局科研基金项目(2006C023)
浙江省教育厅科研项目(20061018)