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黄连解毒汤对小鼠脑缺血慢性神经损伤的保护作用 被引量:13

Protective effects of Huanglian-Jiedu-Tang on chronic brain injury after focal cerebral ischemia in mice
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摘要 目的:观察黄连解毒汤对小鼠脑缺血慢性神经损伤的保护作用。方法:以大脑中动脉阻塞(MCAO)15min诱导小鼠短暂性局灶性脑缺血。从术前7d开始,用药组灌服黄连解毒汤2g/kg和4g/kg,实验对照组和假手术组灌服生理盐水,均连续给药21d,一日一次。缺血后35d(5周)内进行神经症状评分、斜板试验,并记录小鼠的最终生存率。实验结束后,测定脑损伤体积和神经元数量。结果:黄连解毒汤4g/kg能显著提高小鼠MCAO术后35d的最终生存率,2g/kg和4g/kg均明显改善术后的神经症状,降低脑梗死体积、减轻脑萎缩。黄连解毒汤4g/kg可明显增加缺血侧海马CA1区、纹状体和皮层的神经元密度,2g/kg明显增加缺血侧海马CA1区的神经元密度。结论:黄连解毒汤对小鼠局灶性脑缺血慢性神经损伤有保护作用,能提高小鼠的最终生存率并促进神经功能的恢复。 Objective: To investigate the neuroprotective effects of Chinese herb medicine Huanglian-diedu-Tang (HJDT) on chronic brain injury after focal cerebral ischemia in mice. Methods: Focal cerebral ischemia was induced by occlusion of right middle cerebral artery (MCA) for 15 rain. HJDT (at dosage of 2 g/kg or 4 g/kg,qd,orally) was administered for 21 d from d 7 before isehemia until d 14 after ischemia. The sham and ischemic controls were administered with normal saline orally. The neurological deficit scoring and the inclined board testing were performed within 35 d after ischemia. The survival rate,the infarct volume and the neuron density were assessed 35 d after ischemia. Results: HJDT increased the survival rate at dose of 4 g/kg; significantly reduced the neurological deficits,infarct volume and cerebral atrophy at doses of 2 and 4 g/kg after ischemia; and significantly increased the neuron density in the
出处 《浙江大学学报(医学版)》 CAS CSCD 北大核心 2009年第1期75-80,共6页 Journal of Zhejiang University(Medical Sciences)
基金 浙江省中医药管理局科研基金项目(2006C023) 浙江省教育厅科研项目(20061018)
关键词 黄连解毒汤/药理学 脑缺血/中药疗法 脑缺血/病理学 神经元/药物作用 神经元/病理学 HUANGLIAN JIEDU DECOCTION/pharmacol Brain ischemia/ZHONG YAO LIAO FA Brain ischemia/pathol Neurons/drug effe Neurons/pathol
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  • 1XU J H,MURAKAMI Y,MATSUMOTO K,et al. Protective effect of Oren-gedoku to (Huang Lian-Jie-Du-Tang) against impairment of learning and memory induced by transient cerebral ischemia in mice [J]. J Ethnopharmacol,2000,73(3):405-413.
  • 2HWANG Y S, SHIN C Y, HUH Y, et al. Hwangryun Hae-Dok-tang (Huanglian-Jie-Du-Tang ) extract and its constituents reduce ischemia-reperfusion brain injury and neutrophil infiltration in rats [J]. Life Sci,2002,71(18): 2105-2117.
  • 3张艺平 韩鹏.Pharmacody and clinical study on treating encephalopathy by goldthread detoxification decoction .中华实用中西医杂志,2003,3(16):1832-1834.
  • 4徐静华,于庆海,渡边裕司.黄连解毒汤对脑缺血动物的促智作用及机制探讨[J].时珍国医国药,2002,13(12):705-707. 被引量:31
  • 5LID Z, CATHERINE M V,NICOI.E C,et al. L NAME reduces infarction,neurological deficit and blood-brain barrier disruption following cerebral ischemia in mice [J]. Eur J Pharmacol, 2002,457(2-3) : 137-146.
  • 6LONGA E Z,WEINSTEIN P R,CARLSON S, et al. Reversible middle cerebral artery occlusion without craniectomy in rats [J]. Stroke, 1989,20 (1):84-91.
  • 7YONEMORI F, YAMAGUCHI T, YAMADA H, et al. Evaluation of a motor deficit after chronic focal cerebral ischemia in rats [J]. J Cereb Blood Flow Metab, 1998, 18 (10): 1099-1106.
  • 8LIN T N,HE Y Y,WU G,et al. Effect of brain edema on infarct volume in a focal cerebral ischemia model in rats [J]. Stroke,1993,24(1): 117-121.
  • 9RICHARD GREEN A, ODERGREN T, ASHWOOD T. Animal models of stroke:do they have value for discovering neuroproteetive agents? [J]. Trends Pharmacol Sci, 2003, 24 (8) :402-408.
  • 10NAKAYAMA H, JORGENESEN H S, RASHON H, et al. Recovery of upper extremity function in stroke patients: the Copenhagen stroke study [J]. Arch Phys Med Rehabil, 199/1,75 (4) : 394-398.

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