聚乳酸羟基乙酸-泊洛沙姆纳米微粒作为siRNA载体的可行性

Feasibility of PLGA-poloxamer nanoparticle used as siRNA carrier

目的探讨聚乳酸羟基乙酸-泊洛沙姆（PLGA—Poloxamer）纳米微粒作为siRNA载体的可行性。方法乳剂溶解扩散法制备PLGA—Poloxamer纳米微粒，观察纳米微粒的物理特征，探讨不同制备条件对纳米微粒物理特征的影响，测定其对含siRNA片段的质粒DNA的包裹效率，检测释放的质粒DNA的结构稳定性，验证纳米微粒对质粒DNA的保护作用，测定其作为载体的基因转染效率。结果PLGA—Poloxamer纳米微粒呈球形，粒径约为（210．1±24．3）nm，zeta电位为（-0．43±1．43）mv，包裹效率为31％。该微粒对质粒DNA有保护作用，释放的质粒DNA结构稳定，转染效率达28％。结论PLGA—Poloxamer纳米微粒有望成为较理想的siRNA载体。

Objective To investigate the feasibility of PLGA-poloxamer nanoparticle used as siRNA carrier. Methods Nanoparticle was prepared by solvent diffusion technique. The diameter, zeta potential and superficial appearance of nanoparticles were observed. Entrapment efficiency of plasmid DNA, integrity of plasmid DNA released from nanoparticles, DNA protection effect, and transfection efficiency were examined. Results PLGA-poloxamer was a globular particle with diameter about （ 210.1 ± 24.3 ） nm, zeta potential about （ - 0.43 ±1.43 ） mV, and its entrapment efficiency of plasmid DNA was about 31%. Plasmid DNA released from the nanoparticles was almost kept intact. This nanoparticle could protect plasmid against DNase I digestion and the transfection efficiency was 28%. Conclusion PLGA-poloxamer nanoparticle should be considered as an alternation of siRNA carrier.