维生素E抗大鼠四氯化碳所致肝纤维化的实验研究

Effects of vitamin E on carbon tetrachloride-induced hepatic fibrosis-An experimental study

目的为探讨维生素Ｅ（ＶＥ）对肝纤维化的治疗作用。方法观察了ＶＥ对四氯化碳（ＣＣＩ４）诱导的肝纤维化大鼠肝功能，脯氨酸肽酶（ＰＬＤ）、透明质酸（ＨＡ）、丙二醛（ＭＤＡ）、超氧化物歧化酶（ＳＯＤ）及肝脏组织学的影响。同时没有正常对照、溶剂对照及空白对照组。结果维生素Ｅ治疗１０周后，上述生化指标除ＨＡ（３８７．５７±１２９．３９ｎｇ／ｍｌ）仍示达正常外，其余各指标均恢复正常，其中ＡＬＴ于第２周后、ＰＬＤ和ＭＤＡ于第４周后恢复。溶剂对照及空白对照组在第１０周时各指标虽有下降，仍高于ＶＥ治疗组（Ｐ＜０．０１，Ｐ＜０．０５）；光镜观察治疗组大鼠肝内网状纤维由粗大变纤细并有缩短和断裂，经真彩色图像系统分析与对照组比较差异有显著性（Ｐ＜０．０１）。结论ＶＥ治疗有助于实验性四氯化碳肝纤维化的恢复。

Objective To investigate the effects of vitamin E on hepatic fibrosis induced by CCl4. Methods Rats were treated with vitamin E(100mg/kg, twice a week for twenty weeks) after the hepatic fibrosis model was establisbed by nine weeks' CCl4. attack. Serum alanine transaminase (ALT), aspartate transaminase (AST), globulin (GLB), prolidase (PLD), hyaluronic acid (HA), malondialdehyde (MDA) and superoxide dismutase (SOD) were determined periodically. Histopatholog- ical exaznination was carried out at the 10th and 20th weelc. Norznal, solvent and blank control were taken simultaneously , Results All the serum parameters mentioned above were obviously abnormal. Proliferative fibrosis and pseudo-Iobules were observed in the liver. All the parameters except HA(387.57±129.39ng/ml) returned to normal after 10 weeks vitamin E treatment. ALT, PLED and MDA returned to normal at the 2nd, 6th and 4th week respectively. All the parameters of solvent and blank control fell partly but still higher than that of the vitamin E group (p<O.01 or P<0.05). Under light microscopy, collagenous fibers and reticular fibers in the liver tissue changed from a thick form to a slender one, some of which became shorter and broken, These changes were statistically significant as compared with those of the control groups as examined by a real color image analysis system. Conclusion Vitamin E shows good effects on the treatment of hepatic fibrosis induced by CCl4 in rats.