补肾活血法对子宫内膜异位症模型大鼠细胞凋亡的影响

Effect of Reinforcing Kidney and Activating Blood Therapy on Apoptosis of Rat Models with Endometriosis

目的探讨补肾活血法治疗子宫内膜异位症的细胞凋亡机制。方法将雌性8～12周龄SD大鼠分为模型组、西药组、中药组及中西药合并组,每组15只。造模成功后,分别给药4周,采用光镜、电镜、TUNEL法及免疫组化法检测药物干预前后模型大鼠异位子宫内膜的病理形态学、凋亡细胞的阳性率及凋亡调控蛋白bcl-2、bax的阳性表达。结果中西药合并组的异位内膜组织发生凋亡的改变最为明显,凋亡指数最高,并可明显下调bcl-2蛋白的阳性表达和提升bax蛋白阳性的表达,从而使bcl-2/bax比值下降,其次为中药组、西药组和模型组。结论子宫内膜异位症的发生与细胞凋亡能力的下降有关。补肾活血中药能促进异位内膜细胞发生凋亡,使异位病灶萎缩、消退细胞。

Objective To study the mechanism of reinforcing kidney and activating blood therapy for the apoptosis of endometriosis. Methods Female SD rats aged 8-12weeks were grouped as model group, western medicine group, Chinese medicine group and integrated Chinese and western medicine group, with 15 in each group. The light microscope, electron microscope, TUNEL method,and immunohistoche nical method were adopted to observe the pathomorphology, positive rate of apoptotic cells and the positive expression of apoptosis-regulating gene bcl-2 and bax. Results The apoptosis of endometriosis tissues in the integrated Chinese and western medicine group was the most obvious result with the highest apoptosis index, and the positive expression of apoptosis regulating gene bc｜-2 was downward regulated and that of apoptosis regulating gene bax upward regulated, so bcl-2/bax decreased. Then it was Chinese medicine group, western medicine group, and model group. Conclusion The occurrence of endometriosis is related with the lowering of ability of apoptotic cells. Chinese medicines with the functions to reinforce kidney and activate blood can promote the ability of apoptotic ceils in endometriosis to quicken the atrophy and regression of endometriosis tissues.