黄芩素体内抗甲型流感病毒作用的研究

In-vivo Anti-virus Effect of Baicalein on Influenza A Virus

【目的】观察黄芩素的体内抗甲型流感病毒作用。【方法】选用NIH小鼠,随机分为正常对照组,模型组,病毒唑组(剂量为0.07 g.kg-1.d-1),黄芩素高、中、低剂量组(剂量分别为100、10、1μg.kg-1.d-1);采用甲1型流感病毒肺适应株FM1滴鼻感染复制模型后,检测各组肺指数、肺指数抑制率、存活率及死亡保护率,并行肺组织病理检查。【结果】模型组肺指数和存活率显著性降低,与正常对照组比较差异有显著性意义(P<0.01),肺组织出现明显炎性突变;黄芩素高剂量组可显著性升高肺指数和存活率,与模型组比较差异有显著性意义(P<0.05),并可明显改善模型小鼠的肺组织炎性突变,作用与阳性对照药病毒唑相仿。【结论】黄芩素具有一定的体内抗甲型流感病毒作用。

Objective To investigate the anti-virus effect of baicalein on influenza A virus in vivo. Methods NIH mice were randomized into the normal control group, model group, virazole group （ at the dose of 0. 07 g·kg^-1·d^-1） , high-, middle- and low-dose baiealein groups （100, 10 and 1μg·kg^-1·d^-1 respectively）. Mice infected models were induced by intranasal drip of influenza A virus strain FM1. The lung index, inhibitory rate of lung index, survival rate and death prevention rate as well as the pathological changes of lungs were observed. Results Compared with the normal control group, the lung index and survival rate were decreased （P 〈 0. 01 ）, and inflammatory changes were shown in the lungs; the lung index and survival rate were markedly increased in high-dose baiealein group （ P 〈 0.05 compared with the model group） , and the lung inflammatory changes were relieved, the above effects being similar to those of virazole. Conclusion Baiealein exerts certain in-vivo antivirus effect on influenza A virus.