柑桔木质陷孔病类病毒贵州分离物的分子鉴定

Molecular Identification of Guizhou Isolate of Citrus Cachexia Viroid

从采自贵州感染了啤酒花矮化类病毒(Hopstunt viroid,HSVd)的柑桔植株中抽提总核酸,通过一对鉴别引物,经RT-PCR扩增初步筛选出柑桔木质陷孔病类病毒(Citrus cachexia viroid,CCaVd)。扩增该类病毒的全长核苷酸片段,通过克隆和测序,结果发现,该片段全长298 bp,与GenBank中报道的木质陷孔病类病毒分离物同源性达到96%以上。这是中国发生的木质陷孔病类病毒的分子生物学特征的首次报道。

Citrus cachexia viroid （CCaVd） was detected from hop stunt viroid （HSVd） infected citrus collected from Gouzhou by RT - PCR with a discrimination primer pair. The full-length fragment of the CCaVd isolate was amplified by a pair of specific primer. Cloning and sequencing of the fragment revealed it was 298 bp long with above 96% identity compared with reported sequences of CCaVd in GenBank. This is the first report on the molecular characteristics of CCaVd in China.