焊工体内重金属负荷与氧化应激损伤

Effects of Heavy Metals Exposure on Oxidative Stress in Welders

目的研究焊工接触多种重金属与其体内氧化应激损伤的关系。方法采用横断面流行病学调查方法,以123名焊工和82名非焊工为研究对象,以石墨炉原子吸收分光光度法检测血清铅、镉和锰含量,采用超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)评价体内氧化应激水平。结果焊工血铅(115.49±79.22)μg/L和血镉(3.67±3.19)μg/L含量高于对照组;焊工GSH-Px活力(185.4±31.8)U低于对照组,MDA含量(391.0±128.2)μg/L高于对照组。焊工血铅与GSH-Px水平成负相关,与MDA呈正相关;血锰与MDA成正相关。焊工血铅与GSH-Px和MDA的剂量效应关系拟合曲线分别为y=-0.1353x+212.35和y=0.0059x+4.6407。结论氧化应激水平升高是焊工体内过量铅和锰导致神经行为功能改变的机制之一。

Objective To explore the relationship between heavy metals exposure and oxidative stress level in welders. Methods 123 welders and 82 non-welders were investigated by cross-sectional epidemiology. Serum Pb, Cd, and Mn contents were analyzed by graphite AAS. The oxidative stress level was evaluated by SOD, GSH Px and MDA. Results Serum Pb（115. 49±79.22）μg/L and serum Cd（3.67±3.19）μg/L in welders were higher than those of the controls ; while the activities of GSH-Px （ 185.4± 31.8） Ufor welders were lower, and MDA levels（391.0±128.2）μg/L were higher than those of the controls. Serum Pb in welders was negatively correlated with GSH-Px and positively correlated with MDA, Serum Mn in welders was positively correlated ＇with MDA. There existed good dose-effect relationships in-between serum Pb and GSH-Px, and serum Pb and MDA respectively（y= -0. 1353x±212.35 and y=0. 0059x±4. 6407）. Conclusions Elevation of oxidative stress level in welders is resulted by one of the mechanism of neurobehavioral function impairment induced by excessive Pb and Mn exposure.