含银羟基磷灰石种植体的细胞毒性研究

The preparation and biological evalution of Hydroxyapatite/Nanosilver bioceramic coating on Titanium implant surface

目的:在钛种植体表面构筑羟基磷灰石/纳米银涂层并进行生物学评价。方法:采用恒电位电化学法在纯钛表面构建纳米级羟基磷灰石/纳米银(HAp/Nanosilver)涂层。采用扫描电镜(SEM),检测手段对涂层进行理化性能的表征。考察了涂层的结构组分和表面的微观形貌,而后将人牙周膜成纤维样细胞培养于涂层表面,采用细胞毒性评价及其形态观察,分析比较不同表面涂层对牙周膜成纤维样细胞粘附率及增殖率的影响。结果:羟基磷灰石纳米银涂层体外细胞培养显示其具有良好的生物活性。结论:本实验构筑的涂层具有良好的生物相容性和细胞粘附性,应用前景广阔。

Objective： To prepare the composite of Hydroxyapatite / Nanosiliver composite coating on titanium implant surface. Method： A HAp/Nanosiliver composite coating was deposited on the surface of pure titanium by electrochemical deposition. Scanning electromicroscope （SEM） was employed to characterize the obtained coating. The reactions of human pe- riodontal ligament fibroblast-like cells （PDLF） to the coating were also evaluated by MTT assay and SEM observation. Result： The HAp / Nanosiliver bioceramic coating has good crystallization and homogeneousness on nano-scale surface morphology. The PDLF growth was tested by MTT assay and the cell relative growth rate （RGR） was calculated. Conclusion： The HAp / Nanosiliver bioceramic coating on titanium surface possesses good biocompatibility and celluar adhesion and has promising prospects in the future clinical application.